BioBuilder

Author: Natalie Kuldell PhD.
Publisher: "O'Reilly Media, Inc."
ISBN: 1491907533
Format: PDF, ePub, Mobi
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Today’s synthetic biologists are in the early stages of engineering living cells to help treat diseases, sense toxic compounds in the environment, and produce valuable drugs. With this manual, you can be part of it. Based on the BioBuilder curriculum, this valuable book provides open-access, modular, hands-on lessons in synthetic biology for secondary and post-secondary classrooms and laboratories. It also serves as an introduction to the field for science and engineering enthusiasts. Developed at MIT in collaboration with award-winning high school teachers, BioBuilder teaches the foundational ideas of the emerging synthetic biology field, as well as key aspects of biological engineering that researchers are exploring in labs throughout the world. These lessons will empower teachers and students to explore and be part of solving persistent real-world challenges. Learn the fundamentals of biodesign and DNA engineering Explore important ethical issues raised by examples of synthetic biology Investigate the BioBuilder labs that probe the design-build-test cycle Test synthetic living systems designed and built by engineers Measure several variants of an enzyme-generating genetic circuit Model "bacterial photography" that changes a strain’s light sensitivity Build living systems to produce purple or green pigment Optimize baker’s yeast to produce ?-carotene

Analysis II f r Dummies

Author: Mark Zegarelli
Publisher: John Wiley & Sons
ISBN: 3527657983
Format: PDF, ePub, Docs
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Nach der Analysis ist vor der Analysis. Dies ist das richtige Buch f?r Sie, wenn es in der Analysis ein wenig mehr sein soll oder auch muss. Mark Zegarelli erkl?rt Ihnen, was Sie zur infiniten Integration und zu differential- und multivariablen Gleichungen wissen m?ssen. Er f?hrt mit Taylorreihe und Substitutionen fort und f?hrt Sie auch in die Dritte Dimension der Analysis; und das ist lange noch nicht alles! Im Ton verbindlich, in der Sache kompetent f?hrt er Ihre Analysiskenntnisse auf eine neue Stufe.

Genetik f r Dummies

Author: Tara Rodden Robinson
Publisher: John Wiley & Sons
ISBN: 3527811478
Format: PDF, Kindle
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Die Genetik ist eine der Naturwissenschaften, deren Wissen am schnellsten wächst und deren Erkenntnisse ständig in Bewegung und in der Diskussion sind. "Genetik für Dummies" erklärt, was überhaupt hinter diesem spannenden Thema steckt. Die Autorin Tara Rodden Robinson erklärt die Grundlagen der Vererbungslehre wie die Mendelschen regeln, wie Zellen aufgebaut sind und sie sich teilen. Sie zeigt, wie die DNA aufgebaut ist, wie sie kopiert und richtig in Proteine übersetzt wird. Außerdem geht sie auf die Bedeutung der Genetik in der Humanmedizin ein, wie Genmutationen und Erbkrankheiten entstehen. Sie erläutert, was beim Klonen passiert und was sich überhaupt hinter dem Begriff Gentechnik verbirgt. Auch die heißen Themen wie Stammzellentherapie und der Einsatz der Genetik in der Rechtsmedizin werden behandelt.

Synthetic Biology

Author: Josefine Liljeruhm
Publisher: World Scientific
ISBN: 9814579564
Format: PDF, Kindle
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Synthetic Biology: A Lab Manual is the first manual for laboratory work in the new and rapidly expanding field of synthetic biology. Aimed at non-specialists, it details protocols central to synthetic biology in both education and research. In addition, it provides all the information that teachers and students from high schools and tertiary institutions need for a colorful lab course in bacterial synthetic biology using chromoproteins and designer antisense RNAs. As a bonus, practical material is provided for students of the annual international Genetically Engineered Machine (iGEM) competition. The manual is based upon a highly successful course at Sweden's Uppsala University and is coauthored by one of the pioneers of synthetic biology and two bioengineering postgraduate students. An inspiring foreword is written by another pioneer in the field, Harvard's George Church: “Synthetic biology is to early recombinant DNA as a genome is to a gene. Is there anything that SynBio will not impact? There was no doubt that the field of SynBio needed ‘A Lab Manual’ such as the one that you now hold in your hands.” Read about the interview with the authors! Contents:Introduction:What is Synthetic Biology, Exactly?The iGEM OutbreakA Synthetic Biology Lab ManualGenes, Chromoproteins and Antisense RNAs:E. coli DNA: Chromosomes, Plasmids and Copy NumberCoupling of Transcription and Translation in BacteriaPromoter and Terminator for TranscriptionRibosome Binding Site (RBS)Codon BiasChromoproteinsSmall Regulatory RNAs (sRNAs)Lab Rooms and Equipment:The Physical Lab SpacesEquipmentSafety is Priority #1:FiresChemicalsBiological Safety and DisposalDangerous EquipmentLab Course Projects:Time and ResourcesProject Overview and Learning ObjectivesThe Lab NotebookLab Section 1. Preparation of Chemical Solutions and Agar PlatesLab Section 2. Coloring Bacteria by Adding a Promoter to a Chromoprotein GeneLab Section 3: Rational Engineering of Chromoprotein Expression LevelLab Section 4. Other ExperimentsThe “Dreaded” ExamProtocols:IntroductionProtocol 1. Preparation of Solutions and Agar PlatesProtocol 2. Overnight Cultures with Antibiotics, and Glycerol StocksProtocol 3. BioBrick™ 3A Assembly and Gel AnalysisProtocol 4. Agarose Gel ElectrophoresisProtocol 5. Preparation of Competent E. coli Cells Using CaCl2Protocol 6. Transformation of CaCl2-Competent E. coli CellsProtocol 7. Bacterial Re-Streaking TechniquesProtocol 8. Lysis of E. coli Cells with LysozymeProtocol 9. Polymerase Chain Reaction (PCR)Protocol 10. Inverse PCR MutagenesisProtocol 11. Colony PCRProtocol 12. Gibson AssemblyAdvanced Methods:Flow Cytometry and Cell SortingRecombination in Plasmids and the ChromosomeElectrocompetent CellsThe International Genetically Engineered Machine (iGEM) Competition:How to Start an iGEM TeamUppsala iGEM 2011 — Show Color with ColorUppsala iGEM 2012 — Resistance is FutileUppsala iGEM 2013 — Lactonutritious — It's DeliciousAppendices Readership: Students and researchers in biotechnology, cell/molecular biology and genetics. Keywords:Synthetic Biology;Genetic Engineering;DNA Cloning;Polymerase Chain Reaction;Molecular Biology;Laboratory;Biobrick;Chromoprotein;Manual;CourseReviews: It provides an introductory workflow for common techniques and would be of definite use to educators wanting to bring synthetic biology into the classroom or for motivated undergraduate students with the desire to start thir own iGEM team.” Synthetic Biology See Full Review

Mac OS X Leopard f r Dummies

Author: Bob LeVitus
Publisher: John Wiley & Sons
ISBN: 9783527703616
Format: PDF, ePub, Mobi
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Der Leopard zeigt Microsoft die Krallen: Das neue Betriebssystem "Mac OS X Leopard" von Apple stellt nicht nur auf Intel-Chips um, es macht auch einen Riesensatz nach vorn. Die wohl wichtigste der vielen Neuerungen: Boot Camp, das nun in das Betriebssystem integriert ist, unterstützt Windows Vista. Mit Time Machine kann man auch längst gelöschte Dokumente, Fotos oder Songs wieder aus der Versenkung herausholen. Und dass Macs nicht nur praktisch sind, sondern auch ästhetisch ansprechend, beweist "Mail", das neue E-Mail-Programm von Apple, das edel gestylte Vorlagen liefert.

Gentechnologie f r Einsteiger

Author: T. A. Brown
Publisher: Spektrum Akademischer Verlag
ISBN: 9783827428684
Format: PDF, ePub, Docs
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Gentechnologie für Einsteiger hat sich weltweit als leicht verständliche Einführung in dieses wichtige und spannende Wissenschaftsgebiet bewährt. Die sechste Auflage bleibt dem Grundkonzept früherer Auflagen treu, widmet sich aber auch neuen, wachsenden Forschungsfeldern. Das Buch bleibt damit ein unentbehrlicher Leitfaden für Studierende in den Biowissenschaften und ihren zahlreichen Teilgebieten. Es eignet sich auch bestens als Einführung für alle, die sich in ihrem Beruf mit den Grundlagen des Themas vertraut machen müssen.

Non Conventional Copyright

Author: Enrico Bonadio
Publisher: Edward Elgar Publishing
ISBN: 1786434075
Format: PDF, ePub, Docs
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Copyright law constantly evolves to keep up with societal changes and technological advances. Contemporary forms of creativity can threaten the comfortable conceptions of copyright law as creative people continually find new ways of expressing themselves. In this context, Non-Conventional Copyright identifies possible new spaces for copyright protection. With current copyright law in mind, the contributions explore if the law should be more flexible as to whether new or unconventional forms of expression - including graffiti, tattoos, land art, conceptual art and bio art, engineered DNA, sport movements, jokes, magic tricks, DJ sets, 3D printing, works generated by artificial intelligence, perfume making, typefaces, or illegal and immoral works - deserve protection. Vitally, the contributors suggest that it may be time to challenge some of the basic tenets of copyright laws by embracing more flexible ways to identify protectable works and interpret the current requirements for protection. Additionally, some contributors cast doubts about whether copyright is the right instrument to address and regulate these forms of expression. Contemporary in topic, this thought-provoking book will be essential reading for intellectual property law scholars, practitioners and policymakers. Creative people and those involved in the creative industries will also find this book an engaging read.