Colloidal Gold

Author:
Publisher: Elsevier
ISBN: 0323139027
Format: PDF
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Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. Colloidal gold has become the method of choice in immunocytochemistry and many areas of cell biology. This universal method is applicable to most microscopical systems including optical microscopy; scanning, transmission, and high voltage electron microscopy; photoelectron, photon, fluorescent darkfield, and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. This book is among the first available to cover the principles and methodology of colloidal gold in microscopy. Methods are described step by step, to enable researchers to learn these complex procedures solely by reference to these books Problems and limitations of techniques are discussed Guides users to avoid problems and choose the correct procedures for specific applications Contributors are eminent authorities in their fields

Colloidal Gold

Author:
Publisher: Elsevier
ISBN: 0323138810
Format: PDF, ePub, Mobi
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Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today. This universal method is applicable to most microscopical systems including optical microscopy, scanning, transmission and high voltage electron microscopy, and photoelectron, photon, fluorescent darkfield and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. Principles, methods, and applications of colloidal gold methodology in cytochemistry and immunochemistry Methods for preparing colloidal gold particles of different sizes Protein A-gold, protein G-gold, and lectin-gold techniques The use of resins and thin cryosections Multiple labeling

Colloidal Gold

Author: M. A. Hayat
Publisher:
ISBN:
Format: PDF, Kindle
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Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today. This universal method is applicable to most microscopical systems including optical microscopy, scanning, transmission and high voltage electron microscopy, and photoelectron, photon, fluorescent darkfield and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection.

Colloidal Gold

Author: M. A. Hayat
Publisher:
ISBN:
Format: PDF, ePub, Mobi
Download Now
Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. This third volume completes Hayat's coverage of the principles and methodology of colloidal gold in microscopy. The three-volume set should become the standard reference in the field. Key Features * Among the first books to cover principles and methodology of colloidal gold in microscopy * Describes methods step by step to enable researchers to learn these complex procedures solely by reference to the book * Discusses problems and limitations of techniques * Guides users to avoid problems and choose the correct procedures for specific applications * Contributors are eminent authorities in their fields Topics covered in Volume 3 include: * Production of monoclonal and polyclonal antibodies * Lowicryl, polar, and apolar resins * Preserving and localizing antigenicity; sectioning; high-resolution morphological localization * Diaminobenzidine-colloidal gold; microinjection of colloidal gold; double labeling with backscattered electrons; microwave irradiation * Applications to biomedical studies * Colloidal gold in conjunction with video-enhanced microscopy

Colloidal Gold

Author: M. A. Hayat
Publisher:
ISBN:
Format: PDF, Mobi
Download Now
Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today. This universal method is applicable to most microscopical systems including optical microscopy, scanning, transmission and high voltage electron microscopy, and photoelectron, photon, fluorescent darkfield and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection.

Colloid Science

Author: Terence Cosgrove
Publisher: John Wiley & Sons
ISBN: 1444320181
Format: PDF, ePub, Mobi
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Colloidal systems are important across a range of industries, such as the food, pharmaceutical, agrochemical, cosmetics, polymer, paint and oil industries, and form the basis of a wide range of products (eg cosmetics & toiletries, processed foodstuffs and photographic film). A detailed understanding of their formation, control and application is required in those industries, yet many new graduate or postgraduate chemists or chemical engineers have little or no direct experience of colloids. Based on lectures given at the highly successful Bristol Colloid Centre Spring School, Colloid Science: Principles, Methods and Applications provides a thorough introduction to colloid science for industrial chemists, technologists and engineers. Lectures are collated and presented in a coherent and logical text on practical colloid science.

Immunogold Silver Staining

Author: M. A. Hayat
Publisher: CRC Press
ISBN: 9780849324499
Format: PDF, Kindle
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This book discusses the principles, methods, and applications of immunogold-silver staining (IGSS) to biomedical areas. It focuses on the latest advances in the dynamic and progressive field of IGSS.

Immunocytochemistry

Author: Julia M. Polak
Publisher: Butterworth-Heinemann
ISBN: 1483193462
Format: PDF, ePub
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Immunocytochemistry: Practical Applications in Pathology and Biology focuses on the processes, principles, methodologies, and approaches involved in the applications of immunocytochemistry in pathology and biology. The selection first takes a look at the development and scope of immunocytochemistry; techniques and practice used in immunocytochemistry; and raising and testing antibodies for immunocytochemistry. The book then ponders on raising antibodies to small peptides, use of semithin frozen sections in immunocytochemistry, and colloidal gold probes in immunocytochemistry. Discussions focus on the production of gold probes, localization of mouse serum albumin in the mammary gland, immunogens and the immune response, production of antisera, and antibody specificity in immunocytochemistry. The publication takes a look at double immunoenzymatic labelling, lectin histochemistry, immunocytochemical localization of noradrenaline, adrenaline, and serotonin, and immunocytochemistry of cell and tissue cultures. Immunocytochemistry in endocrine pathology and indirect immunofluorescence in the study and diagnosis of organ-specific autoimmune diseases are also discussed. The selection is a dependable source of information for researchers interested the practical applications of immunocytochemistry in pathology and biology.

Functional Polymer Coatings

Author: Limin Wu
Publisher: John Wiley & Sons
ISBN: 1118510704
Format: PDF, Mobi
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Focusing on a variety of coatings, this book provides detailed discussion on preparation, novel techniques, recent developments, and design theories to present the advantages of each function and provide the tools for better product performance and properties. • Presents advantages and benefits of properties and applications of the novel coating types • Includes chapters on specific and novel coatings, like nanocomposite, surface wettability tunable, stimuli-responsive, anti-fouling, antibacterial, self-healing, and structural coloring • Provides detailed discussion on recent developments in the field as well as current and future perspectives • Acts as a guide for polymer and materials researchers in optimizing polymer coating properties and increasing product performance

Materials Syntheses

Author: Ulrich Schubert
Publisher: Springer Science & Business Media
ISBN: 3211751254
Format: PDF, ePub, Mobi
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Materials syntheses are more complex than syntheses of inorganic or organic compounds. Materials synthesis protocols often suffer from unclarities, irreproducibility, lack in detail and lack in standards. The need to change this situation is the main motivation for this book. It collects a number of detailed protocols, ranging from organic polymers to carbonaceous and ceramic materials, from gels to porous and layered materials and from powders and nanoparticles to films.