Colloidal Gold

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Publisher: Elsevier
ISBN: 0323139027
Format: PDF, Docs
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Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. Colloidal gold has become the method of choice in immunocytochemistry and many areas of cell biology. This universal method is applicable to most microscopical systems including optical microscopy; scanning, transmission, and high voltage electron microscopy; photoelectron, photon, fluorescent darkfield, and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. This book is among the first available to cover the principles and methodology of colloidal gold in microscopy. Methods are described step by step, to enable researchers to learn these complex procedures solely by reference to these books Problems and limitations of techniques are discussed Guides users to avoid problems and choose the correct procedures for specific applications Contributors are eminent authorities in their fields

Colloidal Gold

Author: M. A. Hayat
Publisher:
ISBN:
Format: PDF
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Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. This third volume completes Hayat's coverage of the principles and methodology of colloidal gold in microscopy. The three-volume set should become the standard reference in the field. Key Features * Among the first books to cover principles and methodology of colloidal gold in microscopy * Describes methods step by step to enable researchers to learn these complex procedures solely by reference to the book * Discusses problems and limitations of techniques * Guides users to avoid problems and choose the correct procedures for specific applications * Contributors are eminent authorities in their fields Topics covered in Volume 3 include: * Production of monoclonal and polyclonal antibodies * Lowicryl, polar, and apolar resins * Preserving and localizing antigenicity; sectioning; high-resolution morphological localization * Diaminobenzidine-colloidal gold; microinjection of colloidal gold; double labeling with backscattered electrons; microwave irradiation * Applications to biomedical studies * Colloidal gold in conjunction with video-enhanced microscopy

Immunogold Silver Staining

Author: M. A. Hayat
Publisher: CRC Press
ISBN: 9780849324499
Format: PDF, Mobi
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This book discusses the principles, methods, and applications of immunogold-silver staining (IGSS) to biomedical areas. It focuses on the latest advances in the dynamic and progressive field of IGSS.

Practical Electron Microscopy

Author: Elaine Evelyn Hunter
Publisher: Cambridge University Press
ISBN: 9780521385398
Format: PDF, ePub, Mobi
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This is an extensively illustrated laboratory manual of transmission electron microscopy techniques for the laboratory technician, graduate student, or researcher. Chapters begin with a general discussion, move on to the chemicals and equipment required for the method being described and conclude with a step-by-step presentation of the method and instructions for the preparation of solutions. Notes at the end of each chapter warn of possible pitfalls and outline "tricks of the trade". The methods and techniques outlined have been tested for over ten years in clinical and research laboratory situations, and are entirely reliable. Practical Electron Microscopy covers fixation, dehydration and embedding, semithin and thin sectioning, the electron microscope, and photography. For this new edition, the chapters on photography and the electron microscope have been completely rewritten and two new chapters have been added, one on immunoelectron microscopy using colloidal gold, and one dealing with such special techniques as retrieving specimens from paraffin and handling nasal brushings and blood samples.

Immuno Gold Electron Microscopy in Virus Diagnosis and Research

Author: Alexander D. Hyatt
Publisher: CRC Press
ISBN: 9780849367595
Format: PDF, Kindle
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This book presents a wide variety of immuno-gold techniques for use in virus diagnosis and research. Protocols are presented for state-of-the-art techniques, including in situ hybridization, freeze substitution, and the utilization of ultra-small probes and replicas for use by virologists and electron microscopists identifying and studying viruses, their components, and replication in cells. The procedures are described by eminent scientists and are pertinent to both experienced researchers and newcomers to this field who are interested in the localization of low antigenic mass structures.

Biological Electron Microscopy

Author: Michael J. Dykstra
Publisher: Springer Science & Business Media
ISBN: 9780306477492
Format: PDF
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Electron microscopy is frequently portrayed as a discipline that stands alone, separated from molecular biology, light microscopy, physiology, and biochemistry, among other disciplines. It is also presented as a technically demanding discipline operating largely in the sphere of "black boxes" and governed by many absolute laws of procedure. At the introductory level, this portrayal does the discipline and the student a disservice. The instrumentation we use is complex, but ultimately understandable and, more importantly, repairable. The procedures we employ for preparing tissues and cells are not totally understood, but enough information is available to allow investigators to make reasonable choices concerning the best techniques to apply to their parti cular problems. There are countless specialized techniques in the field of electron and light microscopy that require the acquisition of specialized knowledge, particularly for interpretation of results (electron tomography and energy dispersive spectroscopy immediately come to mind), but most laboratories possessing the equipment to effect these approaches have specialists to help the casual user. The advent of computer operated electron microscopes has also broadened access to these instruments, allowing users with little technical knowledge about electron microscope design to quickly become operators. This has been a welcome advance, because earlier instru ments required a level of knowledge about electron optics and vacuum systems to produce optimal photographs and to avoid "crashing" the instruments that typically made it difficult for beginners.

Colloid Science

Author: Terence Cosgrove
Publisher: John Wiley & Sons
ISBN: 1444320181
Format: PDF, Docs
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Colloidal systems are important across a range of industries, suchas the food, pharmaceutical, agrochemical, cosmetics, polymer,paint and oil industries, and form the basis of a wide range ofproducts (eg cosmetics & toiletries, processed foodstuffs andphotographic film). A detailed understanding of their formation,control and application is required in those industries, yet manynew graduate or postgraduate chemists or chemical engineers havelittle or no direct experience of colloids. Based on lectures given at the highly successful Bristol ColloidCentre Spring School, Colloid Science: Principles, Methods andApplications provides a thorough introduction to colloidscience for industrial chemists, technologists and engineers.Lectures are collated and presented in a coherent and logical texton practical colloid science.

Nanomaterials and Nanochemistry

Author: C. Bréchignac
Publisher: Springer Science & Business Media
ISBN: 9783540729938
Format: PDF, ePub
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Here is a brilliant book that covers the major aspects of nanomaterials production. It integrates the many and varied chemical, material and thermo-dynamical facets of production, offering readers a new and unique approach to the subject. The mechanical, optical, and magnetic characteristics of nanomaterials are also presented in detail. Nanomaterials are a fast developing field of research and this book serves as both a reference work for researchers and a textbook for graduate students.

Morphology Methods

Author: Ricardo V. Lloyd
Publisher: Springer Science & Business Media
ISBN: 1592591906
Format: PDF
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The past several decades have witnessed an impressive array of conceptual and techno logical advances in the biomedical sciences. Much of the progress in this area has developed directly as a result of new morphology-based methods that have permitted the assessment of chemical, enzymatic, immunological, and molecular parameters at the cellular and tissue levels. Additional novel approaches including laser capture microdissection have also emerged for the acquisition of homogeneous cell popula tions for molecular analyses. These methodologies have literally reshaped the approaches to fundamental biological questions and have also had a major impact in the area of diagnostic pathology. Much of the groundwork for the development of morphological methods was estab th lished in the early part of the 19 century by Francois-Vincent Raspail, generally acknowledged as the founder of the science of histochemistry. The earliest work in the field was primarily in the hands of botanists and many of the approaches to the under standing of the chemical composition of cells and tissues involved techniques such as microincineration, which destroyed structural integrity. The development of aniline th dyes in the early 20 century served as a major impetus to studies of the structural rather than chemical composition of tissue. Later in the century, however, the focus returned to the identification of chemical constituents in the context of intact cell and tissue structure.

Comprehensive Nanoscience and Technology

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Publisher: Academic Press
ISBN: 9780123743961
Format: PDF, Docs
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From the Introduction: Nanotechnology and its underpinning sciences are progressing with unprecedented rapidity. With technical advances in a variety of nanoscale fabrication and manipulation technologies, the whole topical area is maturing into a vibrant field that is generating new scientific research and a burgeoning range of commercial applications, with an annual market already at the trillion dollar threshold. The means of fabricating and controlling matter on the nanoscale afford striking and unprecedented opportunities to exploit a variety of exotic phenomena such as quantum, nanophotonic and nanoelectromechanical effects. Moreover, researchers are elucidating new perspectives on the electronic and optical properties of matter because of the way that nanoscale materials bridge the disparate theories describing molecules and bulk matter. Surface phenomena also gain a greatly increased significance; even the well-known link between chemical reactivity and surface-to-volume ratio becomes a major determinant of physical properties, when it operates over nanoscale dimensions. Against this background, this comprehensive work is designed to address the need for a dynamic, authoritative and readily accessible source of information, capturing the full breadth of the subject. Its six volumes, covering a broad spectrum of disciplines including material sciences, chemistry, physics and life sciences, have been written and edited by an outstanding team of international experts. Addressing an extensive, cross-disciplinary audience, each chapter aims to cover key developments in a scholarly, readable and critical style, providing an indispensible first point of entry to the literature for scientists and technologists from interdisciplinary fields. The work focuses on the major classes of nanomaterials in terms of their synthesis, structure and applications, reviewing nanomaterials and their respective technologies in well-structured and comprehensive articles with extensive cross-references. It has been a constant surprise and delight to have found, amongst the rapidly escalating number who work in nanoscience and technology, so many highly esteemed authors willing to contribute. Sharing our anticipation of a major addition to the literature, they have also captured the excitement of the field itself in each carefully crafted chapter. Along with our painstaking and meticulous volume editors, full credit for the success of this enterprise must go to these individuals, together with our thanks for (largely) adhering to the given deadlines. Lastly, we record our sincere thanks and appreciation for the skills and professionalism of the numerous Elsevier staff who have been involved in this project, notably Fiona Geraghty, Megan Palmer and Greg Harris, and especially Donna De Weerd-Wilson who has steered it through from its inception. We have greatly enjoyed working with them all, as we have with each other.