Methods in Mycoplasmology V2

Author: Joseph Tully
Publisher: Elsevier
ISBN: 0323149391
Format: PDF
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Methods in Mycoplasmology, Volume II: Diagnostic Mycoplasmology is the second of a two-volume series that resulted from cumulative efforts to meet the need for standardized techniques in mycoplasmology. The book is organized into five sections. Section A discusses techniques for isolating mycoplasmas from humans. These include the recovery of mycoplasmas from the respiratory and genitourinary tracts, from blood and special tissues, and serological Identification of mycoplasmas from humans. Section B focuses on techniques for isolating mycoplasmas from animals, including the recovery and identification of avian, bovine, caprine, ovine, canine, and feline mycoplasmas. Section C presents techniques for isolating mycoplasmas from cell cultures. These include agar and broth techniques, the use of indicator cell lines, and DNA fluorochrome staining. Section D discusses techniques for isolating mycoplasmas from plants and insects. Section E describes some of the techniques that are useful in evaluating the pathogenicity of mycoplasmas, ureaplasmas, and spiroplasmas. Section F defines some of the techniques useful in virus detection by electron microscopy and presents methods for isolating and characterizing viruses from both mycoplasmas and spiroplasmas.

Diagnostic Mycoplasmology

Author: Joseph G. Tully
Publisher:
ISBN:
Format: PDF, Kindle
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Methods in Mycoplasmology, Volume II: Diagnostic Mycoplasmology is the second of a two-volume series that resulted from cumulative efforts to meet the need for standardized techniques in mycoplasmology. The book is organized into five sections. Section A discusses techniques for isolating mycoplasmas from humans. These include the recovery of mycoplasmas from the respiratory and genitourinary tracts, from blood and special tissues, and serological Identification of mycoplasmas from humans. Section B focuses on techniques for isolating mycoplasmas from animals, including the recovery and identi...

Molecular and Diagnostic Procedures in Mycoplasmology

Author:
Publisher: Academic Press
ISBN: 0080536794
Format: PDF, Docs
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This book and its companion, Volume I, concentrate on new procedures--especially those based on the new molecular methodology--developed within the past decade. This volume deals with the new genetic and immunological tools applied to the diagnosis of mycoplasma infections of humans, animals, plants, insects, and all cultures. Volume I outlines the approaches, techniques, and procedures applied to cell and molecular biology studies of mycoplasmas. Key Features * Diagnostic genetic probes * Immunological tools * Antibiotic sensitivity testing * Diagnosis of specific diseases * Experimental infections * Diagnosis of mycoplasma infections of cell cultures

Molecular and Diagnostic Procedures in Mycoplasmology Molecular characterization

Author: Shmuel Razin
Publisher: Elsevier
ISBN: 0125838050
Format: PDF, Mobi
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This volume focuses on new procedures developed since 1985. In particular, it looks at those based on the new molecular methodology responsible for the marked advances in the cell biology of mycoplasmas, and the diagnosis of the infections caused by these organisms. Topics covered include: cultivation and morphology; genome characterization and genetics; membrane characterization; cell metabolism; taxonomy and phylogeny; and pathogenicity.

Methods in Mycoplasmology V1

Author: Samuel Razin
Publisher: Elsevier
ISBN: 0323147135
Format: PDF, Mobi
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Methods in Mycoplasmology, Volume I: Mycoplasma Characterization is the first of a two-volume series that resulted from cumulative efforts to meet the need for standardized techniques in mycoplasmology. The book is organized into five sections. Section A provides an introduction to the mycoplasmas by discussing their characteristics and classification, mycoplasma-animal host interrelationships, and mycoplasma-plant-insect interrelationships. The chapters in Section B examine the morphological and ultrastructural features of mycoplasmas, using techniques such as phase-contrast microscopy, dark-field microscopy, and transmission electron microscopy. Section C discusses the cultivation and nutrition of mycoplasmas. Section D focuses on analytical methods for the characterization of mycoplasmas, including harvest and washing procedures, cell lysis and membrane isolation, and crossed immunoelectrophoresis. Section E describes tests designed to detect specific enzymatic activities or nutritional requirements of mycoplasmas. These include sugar fermentation and arginine hydrolysis tests as well as tests to establish the sterol requirement of mycoplasmas. Section F deals with serological tests. These tests either have specific application to detection of mycoplasma antigens or antibody in plant and animal tissues; are useful in identification of specific groups of mycoplasmas (spiroplasmas); or provide higher sensitivity in measurements of antibodies against mycoplasmas.

Molecular and Diagnostic Procedures in Mycoplasmology Diagnostic procedures

Author: Shmuel Razin
Publisher:
ISBN: 9780125838061
Format: PDF, Kindle
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This is the second of two volumes focusing on new procedures developed between 1985-1995. Topics include: diagnostic genetic probes; immunological tools; antibiotic sensitivity testing; diagnosis of specific diseases; experimental infections; and diagnosis of mycoplasma infections of cell cultures. Other features of the text include: application of molecular probes and PCR as well as new seriological tools in the diagnosis of mycoplasma infections; the new diseases of activation of the immune system by mycoplasmas (serving as superantigens and inducers of cytokines); and the possible association of specific newly discovered mycoplasmas with AIDS.

Molecular Biology and Pathogenicity of Mycoplasmas

Author: Shmuel Razin
Publisher: Springer Science & Business Media
ISBN: 0306476061
Format: PDF, ePub, Docs
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was the result of the efforts of Robert Cleverdon. The rapidly developing discipline of molecular biology and the rapidly expanding knowledge of the PPLO were brought together at this meeting. In addition to the PPLO specialists, the conference invited Julius Marmur to compare PPLO DNA to DNA of other organisms; David Garfinkel, who was one of the first to develop computer models of metabolism; Cyrus Levinthal to talk about coding; and Henry Quastler to discuss information theory constraints on very small cells. The conference was an announcement of the role of PPLO in the fundamental understanding of molecular biology. Looking back 40-some years to the Connecticut meeting, it was a rather bold enterprise. The meeting was international and inter-disciplinary and began a series of important collaborations with influences resonating down to the present. If I may be allowed a personal remark, it was where I first met Shmuel Razin, who has been a leading figure in the emerging mycoplasma research and a good friend. This present volume is in some ways the fulfillment of the promise of that early meeting. It is an example of the collaborative work of scientists in building an understanding of fundamental aspects of biology.

Bergey s Manual of Systematic Bacteriology

Author: Noel R. Krieg
Publisher: Springer Science & Business Media
ISBN: 9780387685724
Format: PDF, Mobi
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Includes a revised taxonomic outline for the phyla Bacteroidetes, Planctomycetes, Chlamydiae, Spirochetes, Fibrobacteres, Fusobacteria, Acidobacteria, Verrucomicrobia, Dictyoglomi, and Gemmatimonadetes based upon the SILVA project as well as a description of more than 153 genera in 29 families. Includes many medically important taxa.

The Mouse in Biomedical Research

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Publisher: Elsevier
ISBN: 9780080469072
Format: PDF, ePub, Docs
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Normative Biology, Husbandry, and Models, the third volume in the four volume set, The Mouse in Biomedical Research, encompasses 23 chapters whose contents provide a broad overview on the laboratory mouse’s normative biology, husbandry, and its use as a model in biomedical research. This consists of chapters on behavior, physiology, reproductive physiology, anatomy, endocrinology, hematology, and clinical chemistry. Other chapters cover management, as well as nutrition, gnotobiotics and disease surveillance. There are also individual chapters describing the mouse as a model for the study of aging, eye research, neurodegenerative diseases, convulsive disorders, diabetes, and cardiovascular and skin diseases. Chapters on imaging techniques and the use of the mouse in assays of biological products are also included.