Directed Evolution of Selective Enzymes

Author: Manfred T. Reetz
Publisher: John Wiley & Sons
ISBN: 3527316604
Format: PDF, ePub, Mobi
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Authored by one of the world's leading organic chemists, this authoritative reference provides an overview of basic strategies in directed evolution and introduces common gene mutagenesis, screening and selection methods. Throughout the text, emphasis is placed on methodology development to maximize efficiency, reliability and speed of the experiments and to provide guidelines for efficient protein engineering. Professor Reetz highlights the application of directed evolution experiments to address limitations in the field of enzyme selectivity, substrate scope, activity and robustness. He critically reviews recent developments and case studies, takes a look at future applications in the field of organic synthesis, and concludes with lessons learned from previous experiments.

Biocatalysis and Agricultural Biotechnology

Author: Ching T. Hou
Publisher: CRC Press
ISBN: 9781420077070
Format: PDF, ePub, Mobi
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Worldwide energy and food crises are spotlighting the importance of bio-based products – an area many are calling on for solutions to these shortages. Biocatalysis and Agricultural Biotechnology encapsulates the cutting-edge advances in the field with contributions from more than 50 international experts comprising sectors of academia, industry, and government research institutes, a virtual Who’s Who among biocatalysis scientists. Created Under the Editorial Guidance of Leading Biotechnology Experts With the aid of numerous graphs and illustrations, this authoritative reference documents such important advances as: Cloning and characterization of Kennedy pathway acyltransferases Engineering of plants for industrial uses New approaches from acquired tolerance to the biotic and abiotic stress of economically important crops This comprehensive text also explores a variety of bio-based industrial products, including: The modification of enzyme character through gene manipulation The biocatalytic synthesis of chiral intermediates for drug development The use of Omega-3 phospholipid nano capsules as effective forms for transporting immune response modifiers Providing in-depth reviews of this ancient field and its modern-day advances, Biocatalysis and Agricultural Biotechnology is an invaluable lab reference for teachers, graduate students, and industrial scientists conducting research in the biosciences.

Enzyme Technologies

Author: Wu-Kuang Yeh
Publisher: John Wiley and Sons
ISBN: 1118125037
Format: PDF, Mobi
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An authoritative review of the latest developments in thechemical biology of enzymes In the first decade of the twenty-first century, enzymes andtheir multiple applications have played a critical role in thediscovery and development of many new therapeutic agents.This bookis a coordinated compilation of research expertise and currentopinion uniquely focused on enzymes and their properties andapplications. Compiled by editors with a combined pharmaceutical experience ofover sixty years, the text provides in-depth reviews of recentdevelopments in selective topics on biosynthesis, biocatalysis, andchemical biology of enzymes as it applies to drug discovery,development, and manufacture. The first in a multi-part series on enzymes, this volumefeatures three sections: New Approaches to Find and Modify Enzymes describes the emergingfield of metagenomics, presents the practical applications ofdirected evolution to enzymes and pathways, and explores approachesfor the discovery and design of biocatalysts Biocatalytic Applications reviews specific applications ofdifferent reactions in producing active pharmaceutical ingredientsand surveys recent developments employing enzymes in organicsynthesis Biosynthetic Applications goes over successful drug discoveriesand developments by combinatorial biosynthesis and reviews theapplication of combinatorial biosynthesis among multiple compatiblehosts These timely discussions, which cover everything from chemicalbiology of enzymes, to the redesign of binding and catalyticspecificities of enzymes, make this volume a valuable tool forkeeping up to date. As such, it is an important read forresearchers, students, and professors in the study ofbiotechnology, life sciences, biochemistry, enzymology, medicinalchemistry, and natural products.

Advances in Catalysis

Author:
Publisher: Elsevier
ISBN: 9780080462189
Format: PDF, Kindle
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Catalysis is the acceleration of a chemical reaction by a catalyst, a substance that notably affects the rate of a chemical reaction without itself being consumed or altered. Since 1948, Advances in Catalysis has filled the gap between the papers that report on and the textbooks that teach in the diverse areas of catalysis research. The editors of and contributors to Advances in Catalysis are dedicated to recording progress in this area. Volume 49 reviews the directed evolution of enantioselective enzymes as catalysts for organic synthesis; dendrimers in catalysis and catalysis in ionic liquids; and the optimization of alkaline earth metal oxide and hydroxide catalysts for base-catalyzed reactions. Provides a comprehensive review of all aspects of catalytic research Contains in-depth, critical, state-of-the-art reports An indispensable source for researchers in academia and industry

Enzyme Functionality

Author: Allan Svendsen
Publisher: CRC Press
ISBN: 0824756916
Format: PDF, ePub, Docs
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Enzyme Functionality serves as a conduit for trailblazing research in enzyme engineering-relating current understanding of sequence families, the new notion of enzyme structure classes, and modern methods in protein engineering, design, and directed evolution to accelerate the development of novel enzyme functionalities. This reference gathers the diverse perspectives of nearly 80 scientists from around the globe and surveys all leading rational and random approaches to the artificial evolution of enzymes. Citing more than 1500 notable works, it outlines assays for enzyme activity, stability, and specificity and a wide variety of site-directed, redesign, and evolutionary engineering methods.

Biocatalysis in the Pharmaceutical and Biotechnology Industries

Author: Ramesh N. Patel
Publisher: CRC Press
ISBN: 1420019376
Format: PDF, ePub, Docs
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Because enzyme-catalyzed reactions exhibit higher enantioselectivity, regioselectivity, substrate specificity, and stability, they require mild conditions to react while prompting higher reaction efficiency and product yields. Biocatalysis in the Pharmaceutical and Biotechnology Industries examines the use of catalysts to produce fine chemicals and chiral intermediates in a variety of pharmaceutical, agrochemical, and other biotechnological applications. Written by internationally recognized scientists in biocatalysis, the authors analyze the synthesis of chiral intermediates for over 60 brand-name pharmaceuticals for a wide range of drug therapies and treatments. From starting material to product, the chapters offer detailed mechanisms that show chiral intermediates and other by-products for each reaction—including hydrolytic, acylation, halogenation, esterification, dehalogenation, oxidation-reduction, oxygenation, hydroxylation, deamination, transamination, and C–C, C–N, C–O bonds formation. Cutting-edge topics include advanced methodologies for gene shuffling and directed evolution of biocatalysts; the custom engineering of enzymes; the use of microbial cells and isolated biocatalysts; the use of renewable starting materials; and generating novel molecules by combinatorial biocatalysis and high-throughput screening. Focusing on industrial applications, the book also considers factors such as bulk processes, instrumentation, solvent selection, and techniques for catalyst immobilization, reusability, and yield optimization throughout. Biocatalysis in the Pharmaceutical and Biotechnology Industries showcases the practical advantages and methodologies for using biocatalysts to develop and produce chiral pharmaceuticals and fine chemicals.

Evolutionary Methods in Biotechnology

Author: Susanne Brakmann
Publisher: John Wiley & Sons
ISBN: 3527604626
Format: PDF, ePub, Mobi
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Miniturization and high throughput assay technology have brought the power of molecular evolution to the bioscience laboratory. Applied wisely, the evolutionary approach can quickly yield the desired result even where other methods have failed. From library generation by random or directed mutagenesis to screening and selection techniques -- the crucial steps for successful evolutionary biotechnology are described in detail in this practical guide that also includes valuable troubleshooting hints on frequently encountered problems. Modern methods for the surface display of peptides and proteins, selective enrichment of nucleic acid aptamers and high-throughput screening of industrial biocatalysts are explained, and computer-based methods for in silico protein and RNA engineering are described as an alternative to in vitro approaches. A special section covers the patenting regulations with regard to biotechnological innovations derived from directed evolution. As an added bonus, a CD-ROM is included that contains software tools for library design, selection of mutagenesis positions, and various predictive algorithms. In short, this practice oriented handbook is an indispensable tool for every scientist working in this interdisciplinary research area.

Asymmetric Organic Synthesis with Enzymes

Author: Vicente Gotor
Publisher: John Wiley & Sons
ISBN: 9783527318254
Format: PDF, Kindle
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Perfect for biochemists, synthetic and organic chemists, this book covers all important reactions, including C-C coupling reactions, oxidation reactions and many more. Divided into two parts, the first section on methodology presents new innovative methods for enzymatic catalysis optimization, including such new trends as medium engineering, directed evolution and computer-aided prediction of enantioselectivity. The second and main section deals with applications to synthesis, showing important reaction types and their applications. Only those reactions with very high selectivity are presented, allowing readers to improve their own reaction yields.