Forensic Applications of High Performance Liquid Chromatography

Author: Shirley Bayne
Publisher: CRC Press
ISBN: 1439882592
Format: PDF, ePub, Docs
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Chromatography has many roles in forensic science, ranging from toxicology to environmental analysis. In particular, high-performance liquid chromatography (HPLC) is a primary method of analysis in many types of laboratories. Maintaining a balance between practical solutions and the theoretical considerations involved in HPLC analysis, Forensic Applications of High Performance Liquid Chromatography uses real-life examples likely to be found within a forensic science laboratory to explain HPLC from a forensic perspective. Focusing chiefly on the reverse phase HPLC mode of separation, this volume examines: The history of HPLC and the theory behind the separation process The requirements for successful analysis and best practice tips The modes of separation and detection most appropriate for forensic science applications HPLC method development and evaluation The quality aspects of laboratory operation Troubleshooting HPLC systems and analyses Applications of HPLC within the field of forensic science Designed as a textbook for university students studying analytical chemistry, applied chemistry, forensic chemistry, or other courses with an element of HPLC within the course curriculum, this volume is also an invaluable guide for those in the early stages of their forensic analysis careers. An instructor’s manual with lecture slides, test bank, objectives, and exercises is available with qualifying course adoption

High Performance Liquid Chromatography

Author: W.J. Lough
Publisher: CRC Press
ISBN: 9780751400762
Format: PDF, ePub, Docs
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High performance liquid chromatography (HPLC) has long been recognized as one of the most useful and versatile analytical techniques. It has now progressed from being a highly expensive method of analysis to a routine technique with wide applications. Consequently there is a requirement in many chemistry and chemistry-related courses for students to acquire a detailed understanding of the principles and practice of HPLC. Written in a manner suitable for undergraduate students studying analytical chemistry and learning about chromatographic analytical techniques applied to pharmaceutical analysis, biochemistry and related disciplines, High-performance Liquid Chromatography: Fundamental Principles and Practice introduces the fundamentals of HPLC. Loosely structured in three parts, the text begins with a thorough introduction of the subject and then progresses through the essential knowledge of the instrumentation needed for HPLC. The final part covers with the applications of HPLC in real-world situations. Developed by a team of international experts from a wide cross-section of disciplines, the text is relevant to a wide range of courses.

Forensic Applications of Gas Chromatography

Author: Michelle Groves Carlin
Publisher: CRC Press
ISBN: 1466507543
Format: PDF, ePub, Mobi
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Several areas of forensic science use the technique of gas chromatography, ranging from fire analysis to the investigation of fraudulent food and perfumes. Covering the essentials of this powerful analytical technique, Forensic Applications of Gas Chromatography explains the theory and shows applications of this knowledge to various realms of forensic science. Topics include: A brief introduction to gas chromatography and its use in forensic science Various components that make up the gas chromatographic instrumentation The theory of the separation process, along with the chemistry underpinning the process Method development, with a specific example of a separation of eight different compounds using a gas chromatography-flame ionization detector Quality assurance and method validation—with information applicable to many types of analytical testing laboratories Troubleshooting in gas chromatography systems New developments in gas chromatography and advances in columns and detectors Real examples supplement the text, along with questions in each chapter. The book includes examples of applications of gas chromatography in drugs, toxicology, fire, paint, food, and fragrance. Each application is presented as an individual case study with specific focus on a particular sample preparation technique. This allows each technique to be discussed with respect to its theory, instrumentation, solvent selection, and function, as appropriate. Each case study provides readers with suitable practical information to allow them to perform experiments in their own laboratory either as part of a practical laboratory class or in a research context. The final chapter provides answers to the questions and encourages further study and discussion.

HPLC in Enzymatic Analysis

Author: Edward F. Rossomando
Publisher: John Wiley & Sons
ISBN: 0470111003
Format: PDF
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The use of High Performance Liquid Chromatography (HPLC) techniques in the study of enzymatic reactions has grown significantly since the publication of the first edition of this highly successful book: the role of enzymes in biological research has expanded; the application of HPLC and enzymes has extended to more disciplines; advances in separation techniques and instrumentation have increased the capability of HPLC; and the discovery of new enzymes has spawned new methods of analysis. High Performance Liquid Chromatography in Enzymatic Analysis, Second Edition addresses these developments in its coverage of the refinements of HPLC methods and their use in a wide range of laboratory applications. It offers the same practical approach found in the first edition, incorporates a wealth of new information into existing chapters, and adds new chapters to deal with new applications, including capillary electrophoresis, forensic chemistry, microdialysis, and the polymerase chain reaction. Topics include: * Application of HPLC to the assay of enzymatic activities * Concepts and principles of HPLC, including the latest technological advances * Concepts and principles of capillary electrophoresis (CE) * Strategy for design of an HPLC/CE system for assay of enzyme activity * Preparation of enzymatic activities from tissues and single cells * Analysis of enzymatic activities in body fluids, including chromatobiosis * HPLC for the identification of new enzymatic activities * Fundamentals of the polymerase chain reaction * HPLC in forensics * Survey of enzymatic activities assayed by the HPLC method, including many new categories * Multienzyme systems, including many new examples * HPLC in the analysis of contaminated food "It is the ability of HPLC to accomplish separations completely and rapidly that led to its original application to problems in the life sciences, particularly those related to purification. An analysis of the literature revealed that this technique was used primarily for the purification of small molecules, macromolecules such as peptides and proteins, and more recently, antibodies. This application to purification has all but dominated the use of the method, and there has been a plethora of books, symposia, and conferences on the use of HPLC for these purposes. However, it was only a matter of time before others began to look beyond and to explore the possibilities that result from the capacity to make separations quickly and efficiently." --from the preface to the First Edition Easy to read and full of practical advice and hundreds of diagrams and examples, High Performance Liquid Chromatography in Enzymatic Analysis, Second Edition is an invaluable resource for students, researchers, and laboratory workers in analytical chemistry and biochemistry, molecular biology and cell biology, and for anyone interested in keeping up with this fast-growing field.

Advances in Food Authenticity Testing

Author: Gerard Downey
Publisher: Woodhead Publishing
ISBN: 0081002335
Format: PDF
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Advances in Food Authenticity Testing covers a topic that is of great importance to both the food industry whose responsibility it is to provide clear and accurate labeling of their products and maintain food safety and the government agencies and organizations that are tasked with the verification of claims of food authenticity. The adulteration of foods with cheaper alternatives has a long history, but the analytical techniques which can be implemented to test for these are ever advancing. The book covers the wide range of methods and techniques utilized in the testing of food authenticity, including new implementations and processes. The first part of the book examines, in detail, the scientific basis and the process of how these techniques are used, while other sections highlight specific examples of the use of these techniques in the testing of various foods. Written by experts in both academia and industry, the book provides the most up-to-date and comprehensive coverage of this important and rapidly progressing field. Covers a topic that is of great importance to both the food industry and the governmental agencies tasked with verifying the safety and authenticity of food products Presents a wide range of methods and techniques utilized in the testing of food authenticity, including new implementations and processes Highlights specific examples of the use of the emerging techniques and testing strategies for various foods

Materials Analysis in Forensic Science

Author: Max M. Houck
Publisher: Academic Press
ISBN: 0128006730
Format: PDF, Docs
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The Advanced Forensic Science Series grew out of the recommendations from the 2009 NAS Report: Strengthening Forensic Science: A Path Forward. This volume, Materials Analysis in Forensic Science will serve as a graduate level text for those studying and teaching materials analysis in forensic science. It will also prove an excellent reference for forensic practitioner’s libraries or use in their casework. Coverage includes methods, textiles, explosives, glass, coatings, geo-and bio-materials, marks and impressions, as well as various other materials and professional issues the reader may encounter. Edited by a world-renowned leading forensic expert, the Advanced Forensic Science Series is a long overdue solution for the forensic science community. Provides basic principles of forensic science and an overview of materials analysis Contains information on a wide variety of trace evidence Covers methods, textiles, explosives, glass, coatings, geo-and bio-materials, marks and impressions, as well as various other materials Includes a section on professional issues, such as: from crime scene to court, lab reports, health and safety, and field deployable devices Incorporates effective pedagogy, key terms, review questions, discussion question and additional reading suggestions

Liquid Chromatography

Author: Salvatore Fanali
Publisher: Elsevier
ISBN: 0128093447
Format: PDF, Kindle
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Liquid Chromatography: Applications, Second Edition,is a single source of authoritative information on all aspects of the practice of modern liquid chromatography. It gives those working in both academia and industry the opportunity to learn, refresh, and deepen their knowledge of the wide variety of applications in the field. In the years since the first edition was published, thousands of papers have been released on new achievements in liquid chromatography, including the development of new stationary phases, improvement of instrumentation, development of theory, and new applications in biomedicine, metabolomics, proteomics, foodomics, pharmaceuticals, and more. This second edition addresses these new developments with updated chapters from the most expert researchers in the field. Emphasizes the integration of chromatographic methods and sample preparation Explains how liquid chromatography is used in different industrial sectors Covers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical) Includes references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision-making

Neuropathology of Drug Addictions and Substance Misuse Volume 2

Author: Victor R. Preedy
Publisher: Academic Press
ISBN: 0128003758
Format: PDF, ePub
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Neuropathology of Drug Addictions and Substance Misuse, Volume 2: Stimulants, Club and Dissociative Drugs, Hallucinogens, Steroids, Inhalants and International Aspects is the second of three volumes in this informative series and offers a comprehensive examination of the adverse consequences of the most common drugs of abuse. Each volume serves to update the reader’s knowledge on the broader field of addiction as well as to deepen understanding of specific addictive substances. Volume 2 addresses stimulants, club and dissociative drugs, hallucinogens, and inhalants and solvents. Each section provides data on the general, molecular and cellular, and structural and functional neurological aspects of a given substance, with a focus on the adverse consequences of addictions. Research shows that the neuropathological features of one addiction are often applicable to those of others, and understanding these commonalties provides a platform for studying specific addictions in more depth and may ultimately lead researchers toward new modes of understanding, causation, prevention, and treatment. However, marshalling data on the complex relationships between addictions is difficult due to the myriad material and substances. Offers a modern approach to understanding the pathology of substances of abuse, offering an evidence-based ethos for understanding the neurology of addictions Fills an existing gap in the literature by serving as a “one-stop-shopping synopsis of everything to do with the neuropathology of drugs of addiction and substance misuse Includes in each chapter: list of abbreviations, abstract, introduction, applications to other addictions and substance misuse, mini-dictionary of terms, summary points, 6+ figures and tables, and full references Offers coverage of preclinical, clinical, and population studies, from the cell to whole organs, and from the genome to whole body

Multidimensional Chromatography

Author: Luigi Mondello
Publisher: John Wiley & Sons
ISBN: 9780471988694
Format: PDF, Mobi
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Coupled High Performance Liquid Chromatography with High Resolution Gas Chromatography (L. Mondello); Multidimensional High Resolution Gas Chromatography (A.C. Lewis); Orthogonal GC-GC (P.J. Marriott); Coupled-Column Liquid Chromatography (C. Corradini); Supercritical Fluid Techniques Coupled with Chromatographic Techniques (F.M. Lancas); Unified Chromatography: Concepts and Considerations for Multidimensional Chromatography (T.L. Chester); Multidimensional Planar Chromatography (Sz. Nyiredy); Multidimensional Electrodriven Separations (M.M. Degen and V.T. Remcho) APPLICATIONS; Foods, Flavours and Fragrances Applications (G. Dugo et al); Biomedical and Pharmaceutical Applications (G.W. Somsen and G.J. de Jong); Industrial and Polymer Applications (Y.V. Kazakevich and R. LoBrutto); Multidimensional Chromatography in Environmental Analysis (R.M. Marce ); Multidimensional Chromatography in the Oil Industry (J. Beens); Forensic and Toxicological Applications.