Forensic DNA Analysis

Author: J. Thomas McClintock
Publisher: CRC Press
ISBN: 1420063308
Format: PDF, Docs
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In its short but active history, the use of DNA typing has revolutionized criminal investigations. It is almost inconceivable to bring a case to trial without positive identification through what is now our most accurate means. Proficiency with the methodology, principles, and interpretation of DNA evidence is crucial for today’s criminalist. An introductory text, Forensic DNA Analysis: A Laboratory Manual presents a contextual history and overview of the science and use of DNA typing. Logically organized, with clear, concise language, this manual provides a fundamental understanding of forensic DNA analysis and a thorough background in the molecular techniques used to determine an individual’s identity. Students are provided with a sound working knowledge of the investigative methodology, scientific principles, and the analysis and interpretation of the resulting data. After laying a foundation on the rules of the laboratory, the basic scientific principles, and the types of biological materials, such as hair, blood, and bone, this practical, hands-on manual provides 12 exercises outlining techniques commonly used in DNA typing. Designed to be performed in a common laboratory, the experiments cover DNA extraction, concentration, and assessment; DNA analysis using restriction fragment length polymorphisms; polymerase chain reaction and PCR-based typing tests; short tandem repeat analysis; and mitochondrial DNA analysis. Many of the procedures described have been adapted from methods used in federal, state, and private forensic laboratories and are suitable to a wide range of applications. There is also an extensive glossary for DNA typing terminology and basic terms used in molecular biology. Instilling confidence, analytical clarity, and a sense of curiosity, this comprehensive introduction is the perfect tool for grasping the techniques and applications of forensic DNA analysis and exploring the questions and issues involved in forensic science investigations.

Forensic DNA Biology

Author: Kelly M. Elkins
Publisher: Academic Press
ISBN: 0123945852
Format: PDF
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A collection of forensic DNA typing laboratory experiments designed for academic and training courses at the collegiate level.

Forensic Analysis of Biological Evidence

Author: J. Thomas McClintock
Publisher: CRC Press
ISBN: 1466504587
Format: PDF
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A powerful tool in the identification of individuals, DNA typing has revolutionized criminal and paternity investigations. Widespread analysis is now conducted by public and private laboratories in the United States and abroad. Focusing on the basic techniques used in forensic DNA laboratories, Forensic Analysis of Biological Evidence: A Laboratory Guide for Serological and DNA Typing introduces readers to the science of serological analysis and DNA typing methods and provides a thorough background of the molecular techniques used to determine an individual’s identity or parental lineage. Originally published as Forensic DNA Analysis: A Laboratory Manual, this revised work offers updated exercises and protocols for all kinds of DNA and serological analyses with delineated objectives, step-by-step procedures, and required laboratory supplies. Each exercise in this manual: Provides an overview of forensic DNA analysis Explains the sources or types of biological material used in a particular DNA analysis Supplies the background principles and practical methodology for specific serological analysis and DNA typing techniques Simulates human forensic testing and can also be used to simulate a wide range of applications for genetic analysis The book contains an extensive glossary to make readers familiar with terminology used in the forensic analysis of biological evidence, as well as basic terms used in molecular biology. Those who master the material in this volume will understand the methodology of the investigation in DNA typing, develop an understanding of the scientific principles involved in serology and DNA analysis, and succeed in analyzing and interpreting the data generated in each exercise with clarity and confidence.

Forensic Biology

Author: Richard Li
Publisher: CRC Press
ISBN: 1420043447
Format: PDF
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Designed as an accessible introduction to basic scientific principles and their application in professional practice, Forensic Biology provides a concise overview of the field. Focusing solely on the science behind the forensic analysis of biological evidence, this book highlights the principles, methods, and techniques used in forensic serologic and forensic DNA analysis. Divided into two areas, the first addresses the identification of biological fluids including blood, semen, and saliva. Chapters instruct on the identification techniques involved in presumptive and confirmatory tests. The second area covers the individualization of biological evidence using forensic DNA techniques. The book demonstrates extraction methods, quantization methods, DNA profiling analysis, and interpretation of results. Each technique introduced in this text is preceded by a brief background of its development and the basic principles that support the technique and its applications. All methods are discussed in detail and accompanied by schematic illustrations where appropriate. Each chapter presents study questions, and references. Instructors have access to a CD containing PowerPoint lecture slides. Emphasizing the fundamentals of basic science and its application to forensic biology, this book provides a solid scientific grounding and familiarity with not just the principles of biological and biochemical processes that occur in forensic analysis, but also the language and vocabulary of forensic biology. The explanations are accessible and straightforward, and informative to facilitate effective learning.

Fundamentals of Forensic DNA Typing

Author: John M. Butler
Publisher: Academic Press
ISBN: 9780080961767
Format: PDF, ePub
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Fundamentals of Forensic DNA Typing is written with a broad viewpoint. It examines the methods of current forensic DNA typing, focusing on short tandem repeats (STRs). It encompasses current forensic DNA analysis methods, as well as biology, technology and genetic interpretation. This book reviews the methods of forensic DNA testing used in the first two decades since early 1980’s, and it offers perspectives on future trends in this field, including new genetic markers and new technologies. Furthermore, it explains the process of DNA testing from collection of samples through DNA extraction, DNA quantitation, DNA amplification, and statistical interpretation. The book also discusses DNA databases, which play an important role in law enforcement investigations. In addition, there is a discussion about ethical concerns in retaining DNA profiles and the issues involved when people use a database to search for close relatives. Students of forensic DNA analysis, forensic scientists, and members of the law enforcement and legal professions who want to know more about STR typing will find this book invaluable. Includes a glossary with over 400 terms for quick reference of unfamiliar terms as well as an acronym guide to decipher the DNA dialect Continues in the style of Forensic DNA Typing, 2e, with high-profile cases addressed in D.N.A.Boxes-- "Data, Notes & Applications" sections throughout Ancillaries include: instructor manual Web site, with tailored set of 1000+ PowerPoint slides (including figures), links to online training websites and a test bank with key

Forensic DNA Profiling Protocols

Author: Patrick J. Lincoln
Publisher: Springer Science & Business Media
ISBN: 0896034437
Format: PDF, ePub, Mobi
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It is now more than ten years since Dr. Alec Jeffreys (now Professor Sir Alec Jeffreys, FRS) reported in Nature that the investigation of certain minisatellite regions in the human genome could produce what he termed DNA fingerprints and provide useful information in the fields of paternity testing and forensic analysis. Since that time we have witnessed a revolution in the field of forensic identification. A total change of technology, from serological or electrophoretic analysis of protein polymorphisms to direct investigation of the underlying DNA polymorphisms has occurred in a short space of time. In addition, the evolution and development of the DNA systems themselves has been rapid and spectacular. In the last decade we have progressed from the multilocus DNA fing- prints, through single locus systems based on the same Southern blot RFLP technology, to a host of systems based on the PCR technique. These include Allele Specific Oligonucleotide (ASO)-primed systems detected by dot blots, the “binary” genotypes produced by mapping variations within VNTR repeats demonstrated by minisatellite variant repeat (MVR) analysis, and yet other fragment-length polymorphisms in the form of Short Tandem Repeat (STR) loci. Hand in hand with the increasing range of systems available has been the development of new instrumentation to facilitate their analysis and allow us to explore the possibilities of high volume testing in the form of mass scre- ing and offender databases.

Inside the Cell

Author: Erin Murphy
Publisher: Nation Books
ISBN: 1568584709
Format: PDF, Mobi
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Josiah Sutton was convicted of rape. He was five inches shorter and 65 pounds lighter than the suspect described by the victim, but at trial a lab analyst testified that his DNA was found at the crime scene. His case looked like many others—arrest, swab, match, conviction. But there was just one problem—Sutton was innocent. We think of DNA forensics as an infallible science that catches the bad guys and exonerates the innocent. But when the science goes rogue, it can lead to a gross miscarriage of justice. Erin Murphy exposes the dark side of forensic DNA testing: crime labs that receive little oversight and produce inconsistent results; prosecutors who push to test smaller and poorer-quality samples, inviting error and bias; law-enforcement officers who compile massive, unregulated, and racially skewed DNA databases; and industry lobbyists who push policies of “stop and spit.” DNA testing is rightly seen as a transformative technological breakthrough, but we should be wary of placing such a powerful weapon in the hands of the same broken criminal justice system that has produced mass incarceration, privileged government interests over personal privacy, and all too often enforced the law in a biased or unjust manner. Inside the Cell exposes the truth about forensic DNA, and shows us what it will take to harness the power of genetic identification in service of accuracy and fairness.

The Basics of Investigating Forensic Science

Author: Kathy Mirakovits
Publisher: CRC Press
ISBN: 1498766692
Format: PDF, ePub, Docs
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Once confined to four-year colleges and graduate schools, forensic science classes can now be found in local high schools as well as in two-year community colleges. The Basics of Investigating Forensic Science: A Laboratory Manual is designed for the beginning forensic science student and for instructors who wish to provide a solid foundation in basic forensic science topics and laboratory techniques. Divided into five distinct sections, the book covers a broad range of subjects, including fingerprinting, shoeprint analysis, firearms, pathology, anthropology, forensic biology, drugs, trace evidence, and more. The book includes extensive notes for instructors to assist in pre-laboratory preparation. Highly illustrated with extensive diagrams and photos, this comprehensive laboratory workbook contains enough pedagogic content to enable it to be used alongside and forensic text or even as a stand-alone text. The laboratory exercises include pre- and post-laboratory questions, illustrating basic crime scene scenarios and clearly stating the objectives of each exercise. Many of the exercises also have additional advanced lab exercises and options for educators with access to more specialized equipment. The Basics of Investigating Forensic Science lends itself to a wide range of academic levels and environments. It is a welcome primer to instructors wanting to conduct experiments, each using essential laboratory techniques, and to address core forensic science concepts.

Practical Forensic Microscopy

Author: Barbara Wheeler
Publisher: John Wiley & Sons
ISBN: 1119965578
Format: PDF, ePub, Docs
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Forensic Microscopy: A Laboratory Manual will provide the student with a practical overview and understanding of the various microscopes and microscopic techniques employed within the field of forensic science. Each laboratory experiment has been carefully designed to cover the variety of evidence disciplines within the forensic science field with carefully set out objectives, explanations of each topic and worksheets to help students compile and analyse their results. The emphasis is placed on the practical aspects of the analysis to enrich student understanding through hands on experience. The experiments move from basic through to specialised and have been developed to cover a variety of evidence disciplines within forensic science field. The emphasis is placed on techniques currently used by trace examiners. This unique, forensic focused, microscopy laboratory manual provides objectives for each topic covered with experiments designed to reinforce what has been learnt along with end of chapter questions, report requirements and numerous references for further reading. Impression evidence such as fingerprints, shoe tread patterns, tool marks and firearms will be analysed using simple stereomicroscopic techniques. Body fluids drug and trace evidence (e.g. paint glass hair fibre) will be covered by a variety of microscopes and specialized microscopic techniques.

Forensic Science

Author: Stuart H. James
Publisher: Taylor & Francis
ISBN: 143985386X
Format: PDF, ePub, Docs
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Covering a range of fundamental topics essential to modern forensic investigation, the fourth edition of the landmark text Forensic Science: An Introduction to Scientific and Investigative Techniques presents contributions from experts in the field who discuss case studies from their own personal files. This edition has been thoroughly updated to reflect the cutting edge of forensic science across many different areas. Designed for a single-term course at the lower undergraduate level, the book begins by discussing the intersection of law and forensic science, how things become evidence, and how courts decide if an item or testimony should be admissible. It takes the evidence from crime scene investigation into laboratory analysis and even onto the autopsy table for the fullest breadth of subject matter of any forensic text available. Topics include Forensic anthropology and the role of entomology in a death investigation Death investigation, including identifying the cause, manner, mechanism, and time of death Bloodstain pattern analysis, the identification of blood and body fluids, the work of forensic toxicologists, and seized drug analysis The history and development of DNA typing and the many ways it can be used Fingerprint, firearm and ballistic, tool mark, tread impression, and trace evidence The forensic analysis of questioned documents and computers Arson, fire, explosives, and the work of forensic engineers in vehicular accidents and structural collapses Forensic psychology and psychiatry, including criminal profiling The future of forensic science Going beyond theory to application, this text incorporates the wisdom of forensic practitioners who discuss the real cases they have investigated. Color-coded sidebars in each chapter provide historical notes, case studies, and current events as well as advice for career advancement. Each section and each chapter begins with an overview and ends with a summary, and key terms, review questions, and up-to-date references are provided. Appropriate for any sensibility, more than 300 photos from real cases give students a true-to-life learning experience. *Access to identical eBook version included