Gene Transfer to Animal Cells

Author: Richard Twyman
Publisher: Garland Science
ISBN: 0203489233
Format: PDF, Kindle
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Gene transfer to animal cells was first achieved more than thirty years ago. Since then, transformation technology has developed rapidly, resulting in a multitude of techniques for cell transformation and the creation of transgenic animals. As with any expanding technology, it becomes difficult to keep track of all the developments and to find a concise and comprehensive source of information that explains all the underlying principles. Gene Transfer to Animals Cells addresses this problem by describing the principles behind gene transfer technologies, how gene expression is controlled in animal cells and how advanced strategies can be used to add, exchange or delete sequences from animal genomes in a conditional manner. A final chapter provides an overview of all the applications of animal cell transformation in farming, medicine and research.

Transgenic Animal Technology

Author: Carl A. Pinkert
Publisher: Newnes
ISBN: 0124095364
Format: PDF, ePub, Mobi
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Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer — from molecular methods to whole animal considerations — for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems. Contains a comprehensive collection of transgenic animal and gene transfer methods Discusses background and introduction to techniques and animal systems Teaches practical step-by-step protocols Fully revised with updates to reflect state-of-the-art technology and associated changes to date

Advanced Biotechnology

Author: R C Dubey
Publisher: S. Chand Publishing
ISBN: 812194290X
Format: PDF, Kindle
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The book embodies 22 chapters covering various important disciplines of biotechnology, such as cell biology, molecular biology, molecular genetics, biophysical methods, genomics and proteomics, metagenomics, enzyme technology, immune-technology, transgenic plants and animals, industrial microbiology and environmental biotechnology. The book is illustrative. It is written in a simple language

Advanced Genetics

Author: Gurbachan S. Miglani
Publisher: CRC Press
ISBN: 9781439892930
Format: PDF, ePub, Docs
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An advanced level volume for postgraduate students and researchers of genetics, cytogenetics biotechnology, biosciences, botany, and zoology which provides detailed coverage of mendelian, molecular, biochemical, immuno, human, mutagenesis, and evolutionary genetics. Concepts, principles and phenomena of genetics have been explained with the help of tables and figures including references, questions and numerical problems at the end of each chapter.

Animal Biotechnology

Author: Committee on Agricultural Biotechnology, Health, and the Environment
Publisher: National Academies Press
ISBN: 0309084393
Format: PDF, ePub
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Genetic-based animal biotechnology has produced new food and pharmaceutical products and promises many more advances to benefit humankind. These exciting prospects are accompanied by considerable unease, however, about matters such as safety and ethics. This book identifies science-based and policy-related concerns about animal biotechnology-key issues that must be resolved before the new breakthroughs can reach their potential. The book includes a short history of the field and provides understandable definitions of terms like cloning. Looking at technologies on the near horizon, the authors discuss what we know and what we fear about their effects-the inadvertent release of dangerous microorganisms, the safety of products derived from biotechnology, the impact of genetically engineered animals on their environment. In addition to these concerns, the book explores animal welfare concerns, and our societal and institutional capacity to manage and regulate the technology and its products. This accessible volume will be important to everyone interested in the implications of the use of animal biotechnology.

Lentivirus Gene Engineering Protocols

Author: Maurizio Federico
Publisher: Springer Science & Business Media
ISBN: 1592593933
Format: PDF, ePub, Mobi
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Maurizio Federico has assembled a panel of outstanding experimenters to detail all the theoretical and practical aspects of lentivirus vector-based gene transfer. The authors demonstrate lentivirus vector production with methods for recovering appropriate producer cells, for producing and titrating lentivirus-containing supernatants, and for detecting transduced cells. The applications of lentivirus vector engineering to different cell types include coverage of lymphocytes, dendritic cells, hematopoietic stem and progenitor cells, mesenchymal stem cells, hippocampal neurons, cardiomyocytes, as well as airway epithelia, corneal cells, and retinal pigment. Also advanced are applications in which genetic material is directly inoculated with lentivirus vectors in such experimental animal models as mouse, rat, and rabbit.

Gene Transfer and Expression in Mammalian Cells

Author: Savvas C. Makrides
Publisher: Gulf Professional Publishing
ISBN: 9780444513700
Format: PDF, ePub, Mobi
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This volume provides a broad, state-of-the-art coverage of diverse technical topics in gene expression in mammalian cells, including the development of vectors for production of proteins in cultured cells, in transgenic animals, vaccination, and gene therapy; progress in methods for the transfer of genes into mammalian cells and the optimization and monitoring of gene expression; advances in our understanding and manipulation of cellular biochemical pathways that have a quantitative and qualitative impact on mammalian gene expression; and the large-scale production and purification of proteins from cultured cells.

Guide to Electroporation and Electrofusion

Author: Donald C. Chang
Publisher: Academic Press
ISBN: 0080917275
Format: PDF, Mobi
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Electroporation is an efficient method to introduce macromolecules such as DNA into a wide variety of cells. Electrofusion results in the fusion of cells and can be used to produce genetic hybrids or hybridoma cells. Guide to Electroporation and Electrofusion is designed to serve the needs of students, experienced researchers, and newcomers to the field. It is a comprehensive manual that presents, in one source, up-to-date, easy-to-follow protocols necessary for efficient electroporation and electrofusion of bacteria, yeast, and plant and animal cells, as well as background information to help users optimize their results through comprehension of the principles behind these techniques. Key Features * Covers fundamentals of electroporation and electrofusion in detail * Molecular events * Mechanisms * Kinetics * Gives extensive practical information * The latest applications * Controlling parameters to maximize efficiency * Available instrumentation * Presents applications of electroporation and electrofusion in current research situations * State-of-the-art modifications to electrical pulses and generators * Application of electroporation and electrofusion to unique, alternative cell and tissue types * Gives straightforward, detailed, easy-to-follow protocols for * Formation of human hybridomas * Introduction of genetic material into plant cells and pollen * Transfection of mammalian cells * Transformation of bacteria, plants, and yeast * Production of altered embryos * Optimization of electroporation by using reporter genes * Comprehensive and up-to-date * Convenient bench-top format * Approximately 125 illustrations complement the text * Complete references with article titles * Written by leading authorities in electroporation and electrofusion

Agricultural Biotechnology

Author: National Research Council (U.S.). Committee on a National Strategy for Biotechnology in Agriculture
Publisher: National Academies Press
ISBN: 030903745X
Format: PDF, ePub, Docs
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Executive summary and recommendations. Scientific aspects. Funding and institutions. Training. Technology transfer.