Gnotobiotic Mouse Technology

Author: Chriss J. Vowles
Publisher: CRC Press
ISBN: 1498736335
Format: PDF, ePub
Download Now
The popularity of germ-free animal models, particularly mice, for investigation of human physiology and disease has recently exploded. Gnotobiotic Mouse Technology: An Illustrated Guide provides the first manual for the maintenance, husbandry, and experimental manipulation of germ-free and gnotobiotic mice. It includes information on all aspects of establishing and operating a germ-free mouse research facility, from basic principles and equipment to detailed instructions for assembling and maintaining isolators, sterilizing supplies, handling animals, and monitoring sterility. The book breaks down techniques and procedures into modules, each of which contains a step-by-step practical and visual guide to a set of related procedures. Each description consists of a materials list, introduction, and general overview, followed by a thoroughly illustrated walkthrough of the steps for each procedure. The descriptions end with troubleshooting tips. Technical chapters are heavily illustrated and include notations of potential pitfalls and alternatives. Other chapters discuss management procedures and practices such as the hiring and training of personnel, setting fee schedules, and record keeping. This book is a landmark resource for establishing and maintaining a facility for germ-free mouse research. Useful to both technicians and investigators, it presents every step necessary to establish a successful facility. It also gives direction in expanding multi-user facilities and applying new technologies to your current practices.

Animal Models for Microbiome Research

Author: National Academies of Sciences, Engineering, and Medicine
Publisher: National Academies Press
ISBN: 0309463912
Format: PDF, ePub, Mobi
Download Now
The surface of the human body and its mucous membranes are heavily colonized by microorganisms. Our understanding of the contributions that complex microbial communities make to health and disease is advancing rapidly. Most microbiome research to date has focused on the mouse as a model organism for delineating the mechanisms that shape the assembly and dynamic operations of microbial communities. However, the mouse is not a perfect surrogate for studying different aspects of the microbiome and how it responds to various environmental and host stimuli, and as a result, researchers have been conducting microbiome studies in other animals. To examine the different animal models researchers employ in microbiome studies and to better understand the strengths and weaknesses of each of these model organisms as they relate to human and nonhuman health and disease, the Roundtable on Science and Welfare in Laboratory Animal Use of the National Academies of Sciences, Engineering, and Medicine convened a workshop in December 2016. The workshop participants explored how to improve the depth and breadth of analysis of microbial communities using various model organisms, the challenges of standardization and biological variability that are inherent in gnotobiotic animal-based research, the predictability and translatability of preclinical studies to humans, and strategies for expanding the infrastructure and tools for conducting studies in these types of models. This publication summarizes the presentations and discussions from the workshop.

Gnotobiotics

Author: Trenton R Schoeb
Publisher: Academic Press
ISBN: 0128045833
Format: PDF, ePub, Mobi
Download Now
Gnotobiotics summarizes and analyzes the research conducted on the use of gnotobiotes, providing detailed information regarding actual facility operation and derivation of gnotobiotic animals. In response to the development of new tools for microbiota and microbiome analysis, the increasing recognition of the various roles of microbiota in health and disease, and the consequent expanding demand for gnotobiotic animals for microbiota/microbiome related research, this volume collates the research of this expanding field into one definitive resource. Reviews and defines gnotobiotic animal species Analyzes microbiota in numerous contexts Presents detailed coverage of the protocols and operation of a gnotobiotic facility

The Gut Microbiome in Health and Disease

Author: Dirk Haller
Publisher: Springer
ISBN: 3319905457
Format: PDF, Docs
Download Now
The book provides an overview on how the gut microbiome contributes to human health. The readers will get profound knowledge on the connection between intestinal microbiota and immune defense systems. The tools of choice to study the ecology of these highly-specialized microorganism communities such as high-throughput sequencing and metagenomic mining will be presented. In addition the most common diseases associated to the composition of the gut flora are discussed in detail. The book will address researchers, clinicians and advanced students working in biomedicine, microbiology and immunology.

Handbook of Radiographic Positioning for Veterinary Technicians

Author: Margi Sirois
Publisher: Cengage Learning
ISBN: 111178180X
Format: PDF
Download Now
The Handbook of Radiographic Positioning for Veterinary Technicians is specifically designed for use as an aid in learning radiographic positioning techniques and as a practical guide for everyday use in the small animal clinic. This concise handbook presents a systematic approach to the positioning of canine, feline, and exotic animal patients for routine and special radiographic procedures. The primary focus is on providing visual aids of animals in position for radiographic procedures. The resulting radiograph produced is included for each radiographic position. A diagram of anatomical landmarks used in determining correct positioning is also included. Important Notice: Media content referenced within the product description or the product text may not be available in the ebook version.

Treatment Planning for Traumatized Teeth

Author: Mitsuhiro Tsukiboshi
Publisher: Quintessence Publishing (IL)
ISBN: 9780867155112
Format: PDF, ePub
Download Now
"Increased knowledge of treatment outcomes, combined with better techniques and materials, has led to improved managemetn and more predictable results for victims of dental trauma. To save teeth and restore function and esthetics, the clinical approach to dental trauma requires accurate diagnosis of the severity and extent of injury, treatment of the acute aspect of the injury, and long-term follow-up and treatment of injury sequelae. Each chapter is arranged by: description; examination and diagnosis; key points; treatment plan; and treatment procedures. Described is the biologic basis of healing and the benefits of allowing healing to be used in the management of traumatic dental injuries. Also described are the roles orthodontic and restorative modalities play in the management of dental trauma. More than 50 cases and 480 images&—intraoral, radiographic, and schematic&—elucidate the most effective approaches to all forms of dental trauma."--Publisher.

Laboratory Animal Medicine

Author: Lynn C. Anderson
Publisher: Elsevier
ISBN: 012416613X
Format: PDF, ePub, Docs
Download Now
Laboratory Animal Medicine, Third Edition, is a fully revised publication from the American College of Laboratory Medicine’s acclaimed blue book series. It presents an up-to-date volume that offers the most thorough coverage of the biology, health, and care of laboratory animals. The book is organized by species, with new inclusions of chinchillas, birds, and program and employee management, and is written and edited by known experts in the fields. Users will find gold-standard guidance on the study of laboratory animal science, as well as valuable information that applies across all of the biological and biomedical sciences that work with animals. Organized by species for in-depth understanding of biology, health, and best care of animals Features the inclusion of chinchillas, quail, and zebra finches as animal models Offers guidance on program and employee management Covers regulations, policies, and laws for laboratory animal management worldwide

Epigenetics and Cancer

Author: Stephen F. Goodwin
Publisher: Academic Press
ISBN: 9780123808646
Format: PDF, ePub, Docs
Download Now
Genes interact with the environment, experience, and biology of the brain to shape an animal's behavior. This latest volume in Advances in Genetics, organized according to the most widely used model organisms, describes the latest genetic discoveries in relation to neural circuit development and activity. * Explores the latest topics in neural circuits and behavior research in zebrafish, drosophila, C.elegans, and mouse models * Includes methods for testing with ethical, legal, and social implications * Critically analyzes future prospects

The New Science of Metagenomics

Author: Committee on Metagenomics: Challenges and Functional Applications
Publisher: National Academies Press
ISBN: 0309106761
Format: PDF, Mobi
Download Now
Although we can't usually see them, microbes are essential for every part of human life -- indeed all life on Earth. The emerging field of metagenomics offers a new way of exploring the microbial world that will transform modern microbiology and lead to practical applications in medicine, agriculture, alternative energy, environmental remediation, and many others areas. Metagenomics allows researchers to look at the genomes of all of the microbes in an environment at once, providing a "meta" view of the whole microbial community and the complex interactions within it. It's a quantum leap beyond traditional research techniques that rely on studying -- one at a time -- the few microbes that can be grown in the laboratory. At the request of the National Science Foundation, five Institutes of the National Institutes of Health, and the Department of Energy, the National Research Council organized a committee to address the current state of metagenomics and identify obstacles current researchers are facing in order to determine how to best support the field and encourage its success. The New Science of Metagenomics recommends the establishment of a "Global Metagenomics Initiative" comprising a small number of large-scale metagenomics projects as well as many medium- and small-scale projects to advance the technology and develop the standard practices needed to advance the field. The report also addresses database needs, methodological challenges, and the importance of interdisciplinary collaboration in supporting this new field.

Small Animal Imaging

Author: Fabian Kiessling
Publisher: Springer
ISBN: 3319422022
Format: PDF, ePub
Download Now
This textbook is a practical guide to the use of small animal imaging in preclinical research that will assist in the choice of imaging modality and contrast agent and in study design, experimental setup, and data evaluation. All established imaging modalities are discussed in detail, with the assistance of numerous informative illustrations. While the focus of the new edition remains on practical basics, it has been updated to encompass a variety of emerging imaging modalities, methods, and applications. Additional useful hints are also supplied on the installation of a small animal unit, study planning, animal handling, and cost-effective performance of small animal imaging. Cross-calibration methods and data postprocessing are considered in depth. This new edition of Small Animal Imaging will be an invaluable aid for researchers, students, and technicians involved in research into and applications of small animal imaging.