Handbook of Single Molecule Biophysics

Author: Peter Hinterdorfer
Publisher: Springer Science & Business Media
ISBN: 9780387764979
Format: PDF
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This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.

Handbook of Single Molecule Fluorescence Spectroscopy

Author: Chris Gell
Publisher: Oxford University Press on Demand
ISBN: 0198529422
Format: PDF, Docs
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This is a practical introduction to single molecule fluorescence experiments, the analysis of the data, and applications of the techniques to the study of biological structure and function.

Handbook of Fluorescence Spectroscopy and Imaging

Author: Markus Sauer
Publisher: John Wiley & Sons
ISBN: 3527633529
Format: PDF
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Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.

Handbook of Molecular Force Spectroscopy

Author: Aleksandr Noy
Publisher: Springer Science & Business Media
ISBN: 038749989X
Format: PDF, ePub, Mobi
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Researchers in academia and industry who are interested in techniques for measuring intermolecular forces will find this an essential text. It presents a review of modern force spectroscopy, including fundamentals of intermolecular forces, technical aspects of the force measurements, and practical applications. The handbook begins with a review of the fundamental physics of loading single and multiple chemical bonds on the nanometer scale. It contains a discussion of thermodynamic and kinetic models of binding forces and dissipation effects in nanoscale molecular contacts, covers practical aspects of modern single-molecule level techniques, and concludes with applications of force spectroscopy to chemical and biological processes. Computer modeling of force spectroscopy experiments is also addressed.

Handbook of Single Molecule Electronics

Author: Kasper Moth-Poulsen
Publisher: CRC Press
ISBN: 9814463396
Format: PDF, Kindle
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Single-molecule electronics has evolved as a vibrant research field during the last two decades. The vision is to be able to create electronic components at the highest level of miniaturization—the single molecule. This book compiles and details cutting-edge research with contributions from chemists, physicists, theoreticians, and engineers. It covers all aspects of single-molecule electronics, from the theory through experimental realizations and the chemical synthesis of molecular components to the implementation of molecular components in future integrated circuits. This book describes in detail both established methods and recent advances in the field, including vibrational effects, switching phenomena, quantum interference, thermal power, and parallel assembly strategies. The authors add more details to the chapters than typically found in the primary literature so that the book can be read not only by specialists but also by non-experts and students with an interest in the research field. Each chapter is accompanied by problems, and a solutions manual is also provided.

Single Particle Tracking and Single Molecule Energy Transfer

Author: Christoph Bräuchle
Publisher: John Wiley & Sons
ISBN: 9783527628377
Format: PDF
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Closing a gap in the literature, this handbook gathers all the information on single particle tracking and single molecule energy transfer. It covers all aspects of this hot and modern topic, from detecting virus entry to membrane diffusion, and from protein folding using spFRET to coupled dye systems, as well recent achievements in the field. Throughout, the first-class editors and top international authors present content of the highest quality, making this a must-have for physical chemists, spectroscopists, molecular physicists and biochemists.

Handbook of Dynein

Author: Keiko Hirose
Publisher: CRC Press
ISBN: 981430333X
Format: PDF, Docs
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This book draws together recent data on both cytoplasmic and flagellar dyneins and the proteins they interact with, to give readers a clear picture of what is currently known about the structure and mechanics of these remarkable macro-molecular machines. Each chapter is written by active researchers, with a focus on currently used biophysical, biochemical, and cell biological methods. This is a useful handbook for frontline researchers as well as a textbook for advanced students. Besides comprehensive cover of structural information gained by electron microscopy, electron cryo-tomography, X-ray crystallography, and nuclear magnetic resonance, this book provides detailed descriptions of mechanistic experiments by single-molecule nanometry. The reports include in vitro studies of the motility of reconstituted complexes and in vivo studies of organisms expressing mutant dyneins. The recent technical improvements described, which have played an important part in recent advances, include the expression and preparation of recombinant dynein heavy chains or individual subdomains.

Biomembrane Frontiers

Author: Thomas Jue
Publisher: Springer Science & Business Media
ISBN: 160761314X
Format: PDF, Kindle
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This is the second book in the Handbook of Modern Biophysics series, dedicated to fundamental topics and new applications in biophysics. This book on biomembranes covers theory and application and includes problem sets, references and guides for further study.

Biophysics

Author: William Bialek
Publisher: Princeton University Press
ISBN: 1400845572
Format: PDF, Mobi
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Interactions between the fields of physics and biology reach back over a century, and some of the most significant developments in biology--from the discovery of DNA's structure to imaging of the human brain--have involved collaboration across this disciplinary boundary. For a new generation of physicists, the phenomena of life pose exciting challenges to physics itself, and biophysics has emerged as an important subfield of this discipline. Here, William Bialek provides the first graduate-level introduction to biophysics aimed at physics students. Bialek begins by exploring how photon counting in vision offers important lessons about the opportunities for quantitative, physics-style experiments on diverse biological phenomena. He draws from these lessons three general physical principles--the importance of noise, the need to understand the extraordinary performance of living systems without appealing to finely tuned parameters, and the critical role of the representation and flow of information in the business of life. Bialek then applies these principles to a broad range of phenomena, including the control of gene expression, perception and memory, protein folding, the mechanics of the inner ear, the dynamics of biochemical reactions, and pattern formation in developing embryos. Featuring numerous problems and exercises throughout, Biophysics emphasizes the unifying power of abstract physical principles to motivate new and novel experiments on biological systems. Covers a range of biological phenomena from the physicist's perspective Features 200 problems Draws on statistical mechanics, quantum mechanics, and related mathematical concepts Includes an annotated bibliography and detailed appendixes Instructor's manual (available only to teachers)

Biomolecular Kinetics

Author: Clive R. Bagshaw
Publisher: CRC Press
ISBN: 1351646664
Format: PDF, ePub, Docs
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"a gem of a textbook which manages to produce a genuinely fresh, concise yet comprehensive guide" –Mark Leake, University of York "destined to become a standard reference.... Not just a ‘how to’ handbook but also an accessible primer in the essentials of kinetic theory and practice." –Michael Geeves, University of Kent "covers the entire spectrum of approaches, from the traditional steady state methods to a thorough account of transient kinetics and rapid reaction techniques, and then on to the new single molecule techniques" –Stephen Halford, University of Bristol This illustrated treatment explains the methods used for measuring how much a reaction gets speeded up, as well as the framework for solving problems such as ligand binding and macromolecular folding, using the step-by-step approach of numerical integration. It is a thoroughly modern text, reflecting the recent ability to observe reactions at the single-molecule level, as well as advances in microfluidics which have given rise to femtoscale studies. Kinetics is more important now than ever, and this book is a vibrant and approachable entry for anyone who wants to understand mechanism using transient or single molecule kinetics without getting bogged down in advanced mathematics. Clive R. Bagshaw is Emeritus Professor at the University of Leicester, U.K., and Research Associate at the University of California at Santa Cruz, U.S.A.