Metabolomics in Practice

Author: Michael Lämmerhofer
Publisher: John Wiley & Sons
ISBN: 3527655883
Format: PDF, Kindle
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Unlike other handbooks in this emerging field, this guide focuses on the challenges and critical parameters in running a metabolomics study, including such often-neglected issues as sample preparation, choice of separation and detection method, recording and evaluating data as well as method validation. By systematically covering the entire workflow, from sample preparation to data processing, the insight and advice offered here helps to clear the hurdles in setting up and running a successful analysis, resulting in high-quality data from every experiment. Based on more than a decade of practical experience in developing, optimizing and validating metabolomics approaches as a routine technology in the academic and industrial research laboratory, the lessons taught here are highly relevant for all systems-level approaches, whether in systems biology, biotechnology, toxicology or pharmaceutical sciences. From the Contents: * Sampling and Sample Preparation in Microbial Metabolomics * Tandem Mass Spectrometry Hyphenated with HPLC and UHPLC for Targeted Metabolomics * GC-MS, LC-MS, CE-MS and Ultrahigh Resolution MS (FTICR-MS) in Metabolomics * NMR-based metabolomics analysis * Potential of Microfluidics and Single Cell Analysis in Metabolomics * Data Processing in Metabolomics * Validation and Measurement Uncertainty in Metabolomic Studies * Metabolomics and its Role in the Study of Mammalian Systems and in Plant Sciences * Metabolomics in Biotechnology and Nutritional Metabolomics and more.

Comprehensive Analysis of Parasite Biology

Author: Sylke MÃ1⁄4ller
Publisher: John Wiley & Sons
ISBN: 3527694110
Format: PDF, Docs
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Written and edited by experts in the field, this book brings together the current state of the art in phenotypic and rational, target-based approaches to drug discovery against pathogenic protozoa. The chapters focus particularly on virtual compounds and high throughput screening, natural products, computer-assisted drug design, structure-based drug design, mechanism of action identification, and pathway modelling. Furthermore, state-of the art "omics" technologies are described and currently studied enzymatic drug targets are discussed. Mathematical, systems biology-based approaches are introduced as new methodologies for dissecting complex aspects of pathogen survival mechanisms and for target identification. In addition, recently developed anti-parasitic agents targeting particular pathways, which serve as lead compounds for further drug development, are presented.

Botanicals

Author: Kurt Reynertson
Publisher: CRC Press
ISBN: 1466598425
Format: PDF, Mobi
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The international trade in plants is growing steadily as the worldwide demand for natural and botanical raw materials increases. Customers value natural products and botanicals as "green" alternatives—safer ingredients for their families which also represent an environmentally and socially responsible choice for the planet. In order to build assurance into the sourcing of natural ingredients, R&D organizations must have valid scientific matrices to authenticate the quality of those ingredients, provide traceability, and minimize risk. An assemblage of insight from expert contributors, Botanicals: Methods and Techniques for Quality & Authenticity compiles a range of methods and techniques that can be used to help guide quality and authenticity determinations. Topics include: Metabolic profiling, authentication of botanicals by morphology, and genetic methods of botanical authentication Tools for building models for the authentication of materials How multivariate statistics can play a role in determining botanical quality and authenticity Radiocarbon and stable isotope ratio analysis and emerging stable isotope tools NMR (nuclear magnetic resonance) spectroscopy, NIR (near-infrared), and HPTLC (high-performance thin-layer chromatography) methods for analysis The use of electronic sensing instruments and applications for analysis The contributors also discuss the challenge of identifying a botanical extract or preparation on the basis of its chemical content and discuss quality issues faced by botanicals used as cosmetic ingredients. The book provides you with a range of traditional, taxonomic, and newer analytical tools to assure the quality, authenticity, and traceability of botanical raw materials for dietary supplements, cosmetics, and natural products research.

Metabolomics Metabonomics and Metabolite Profiling

Author: William J. Griffiths
Publisher: Royal Society of Chemistry
ISBN: 0854042997
Format: PDF, Mobi
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In this comprehensive resource the Editor draws together experts from the field and provides an insightful introduction into the technology and methodology.

Metabolomics

Author: Wolfram Weckwerth
Publisher: Springer Science & Business Media
ISBN: 1597452440
Format: PDF, ePub, Docs
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Metabolomics: Methods and Protocols examines the state-of-the-art in metabolomic analysis. Leading researchers in the field present protocols for the application of complementary analytical methods, such as gas chromatography-mass spectrometry (GC-MS). Metabolomics: Methods and Protocols contains forward-looking protocols, which provide the essential groundwork for future efforts in elucidating the structure of the unknowns detected in metabolomic studies.

Metabolomics From Fundamentals to Clinical Applications

Author: Alessandra Sussulini
Publisher: Springer
ISBN: 3319476564
Format: PDF, ePub, Mobi
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This book provides a comprehensive view of metabolomics, from the basic concepts, through sample preparation and analytical methodologies, to data interpretation and applications in medicine. It is the first volume to cover metabolomics clinical applications while also emphasizing analytical and statistical features. Moreover, future trends and perspectives in clinical metabolomics are also presented. For researches already experienced in metabolomics, the book will be useful as an updated definitive reference. For beginners in the field and graduate students, the book will provide detailed information about concepts and experimental aspects in metabolomics, as well as examples and perspectives of applications of this strategy to clinical questions.

Metabolic Profiling

Author: Georgios A. Theodoridis
Publisher: Humana Press
ISBN: 9781493976423
Format: PDF, Kindle
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This volume explores the different approaches and techniques used by researchers to study the recent challenges and developments in metabolic profiling. This book is divided into IV parts. Part I contains chapters that highlight basic concepts, such as experimental design, data treatment, metabolite identification, and harmonization. Part II describes experimental protocols for both targeted and untargeted metabolomics covering the basic analytical technologies: LC-MS, GC-MS, NMR and CE-MS. In addition the protocols describe methods for the study of tissues, feces, blood and other types of biological samples as well as the application of chemical derivatization for GC-MS. Parts III and IV present the use of metabolomics in the study of food, plants and the life sciences, with examples from the quest for the discovery of disease biomarkers, physical exercise omics and metabolic profiling of food, fruit and wine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Metabolic Profiling: Methods and Protocols is a valuable resource for researchers who are interested in expanding their knowledge of this rapidly developing field.

The Handbook of Plant Metabolomics

Author: Wolfram Weckwerth
Publisher: John Wiley & Sons
ISBN: 3527669906
Format: PDF, Docs
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This is the newest title in the successful Molecular Plant Biology Handbook Series. Just like the other titles in the series this new book presents an excellent overview of different approaches and techniques in Metabolomics. Contributors are either from ivy-league research institutions or from companies developing new technologies in this dynamic and fast-growing field. With its approach to introduce current techniques in plant metabolomics to a wider audience and with many labs and companies considering to introduce metabolomics for their research, the title meets a growing market. The Kahl books are in addition a trusted brand for the plant science community and have always sold above expectations.

NMR based Metabolomics

Author: Hector C Keun
Publisher: Royal Society of Chemistry
ISBN: 184973643X
Format: PDF, Mobi
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This book describes the state of the art in the application of NMR spectroscopy to metabolomics and will be a key title for researchers and practitioners.

Methodologies for Metabolomics

Author: Norbert W. Lutz
Publisher: Cambridge University Press
ISBN: 1139619055
Format: PDF
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Metabolomics, the global characterisation of the small molecule complement involved in metabolism, has evolved into a powerful suite of approaches for understanding the global physiological and pathological processes occurring in biological organisms. The diversity of metabolites, the wide range of metabolic pathways and their divergent biological contexts require a range of methodological strategies and techniques. Methodologies for Metabolomics provides a comprehensive description of the newest methodological approaches in metabolomic research. The most important technologies used to identify and quantify metabolites, including nuclear magnetic resonance and mass spectrometry, are highlighted. The integration of these techniques with classical biological methods is also addressed. Furthermore, the book presents statistical and chemometric methods for evaluation of the resultant data. The broad spectrum of topics includes a vast variety of organisms, samples and diseases, ranging from in vivo metabolomics in humans and animals to in vitro analysis of tissue samples, cultured cells and biofluids.