Plant Tissue Culture

Author: Roberta H. Smith
Publisher: Academic Press
ISBN: 0124159206
Format: PDF, Mobi
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Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems Includes detailed drawings that complement both introductions and experiments Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology Fully updated literature

Experiments in Plant Tissue Culture

Author: John H. Dodds
Publisher: International Potato Center
ISBN: 9780521315166
Format: PDF, Kindle
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The second edition of Experiments in Plant Tissue Culture makes available new information that has resulted from recent advances in the applications of plant tissue culture techniques to agriculture and industry. This comprehensive laboratory text takes the reader through a graded series of experimental protocols and also provides an introductory review of each topic. Topics include: a plant tissue culture laboratory, aseptic techniques, nutritional components of media, callus induction, organ formation, xylem cell differentiation, root cultures, cell suspensions, micropropagation, embryogenesis, isolation and fusion of protoplasts, haploid cultures, storage of plant genetic resources, secondary metabolite production, and quantification of procedures. This volume offers all of the basic experimental methods for the major research areas of plant tissue culture, and it will be invaluable to undergraduates and research investigators in the plant sciences.

Experiments in Plant Tissue Culture

Author: John H. Dodds
Publisher: Cambridge University Press
ISBN: 9780521299657
Format: PDF
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The second edition of Experiments in Plant Tissue Culture makes available new information that has resulted from recent advances in the applications of plant tissue culture techniques to agriculture and industry. This comprehensive laboratory text takes the reader through a graded series of experimental protocols and also provides an introductory review of each topic. Topics include: a plant tissue culture laboratory, aseptic techniques, nutritional components of media, callus induction, organ formation, xylem cell differentiation, root cultures, cell suspensions, micropropagation, embryogenesis, isolation and fusion of protoplasts, haploid cultures, storage of plant genetic resources, secondary metabolite production, and quantification of procedures. This volume offers all of the basic experimental methods for the major research areas of plant tissue culture, and it will be invaluable to undergraduates and research investigators in the plant sciences.

Plant Tissue Culture

Author: B. N. Sathyanarayana
Publisher: I. K. International Pvt Ltd
ISBN: 8189866117
Format: PDF, Kindle
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Practices and New Experimental Protocols is being brought out to fill the existing gap in the available literature on plant tissue culture, especially focusing on the aspects of practical procedures and protocols of tissue culture. This book contains important experimental techniques and gives guidance on carrying out hands-on experiences. It has been designed in a simple way, giving all the necessary procedures as a general guideline and also necessary tips to maneuver any problem encountered. These tips are based on the first hand experiences of the author while teaching and researching the techniques of plant tissue culture. A unique feature of this book is the inclusion of several techniques describing the actual protocols experimented and developed with different plant species by different scientists. A substantial number of original colored plates including fluorescence photographs standout the book. This pioneering work is valuable for the students who are looking for fresh outlook and search.

Plant Tissue Culture Theory and Techniques

Author: Shailesh Kumar
Publisher: Scientific Publishers
ISBN: 938610220X
Format: PDF, Kindle
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Biotechnology is an emerging field of science and as such the government of India is laying a large and exclusive impetus on it. Plant tissue culture is the basic and the most important aspect of Biotechnology. Therefore, plant tissue culture has been introduced as a compulsory course in the Undergraduate and Postgraduate syllabi of all the Agricultural Universities, ICAR institutes and other plant science related educational organizations. This book has been designed to benefit the students, the research scholars and the scientists for developing a level of self-confidence to conduct the experiments independently and can acquire the practical skills along with the basic know-how about the techniques being used. Each chapter is devoted to a separate aspect of plant tissue culture and the chapters are arranged in the order of increasing technical complexity. The opening chapters present a brief historical survey of the field of plant tissue culture, a background in sterilization techniques. The text deals with the experimental details of each and every technique. The protocols have been simplified legibly to include details and notes that we hope will help the user avoid unnecessary errors and confusion. All the applications of plant tissue culture have been very well discussed and the techniques associated with them described in detail. This being a comp-lete book on Plant tissue culture will solve all types of problem of the users who will not have to use other resource books for the same purpose.

Experiments in Microbiology Plant Pathology and Biotechnology

Author: K. R. Aneja
Publisher: New Age International
ISBN: 9788122414943
Format: PDF
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Microorganisms Are Living Things Like Plants And Animals But Because Of Their Minute Size And Omnipresence, Performing Experiments With Microbes Requires Special Techniques And Equipment Apart From Good Theoretical Knowledge About Them. This Easy To Use Revised And Updated Edition Provides Knowledge About All The Three I.E., Techniques, Equipment And Principles Involved.The Notable Feature Of This Edition Is The Addition Of New Sections On Bacterial Taxonomy That Deals With The Criteria Used In Identification, Phylogeny And Current System Of Classification Of Procaryotes Based On The Second Edition Of Bergey Manual Of Systematic Bacteriology And The Section One On History Of Discovery Of Events That Covers Chronologically Important Events In Microbiology With The Contribution Of Pioneer Microbiologists Who Laid The Foundation Of The Science Of Microbiology. In The Subsequent Twenty-Two Sections, Various Microbiological Techniques Have Been Described Followed By Several Experiments Illustrating The Properties Of Microorganisms And Highlighting Their Involvement In Practically Every Sphere Of Life.Along With The Cultivation/Isolation/Purification Of Microbes, This Edition Also Contains Exercises Concerning Air, Soil, Water, Food, Dairy And AgriculturalMicrobiology, Bacterial Genetics, Plant Pathology, Plant Tissue Culture And Mushroom Production Technology. This Manual Contains 163 Experiments Spread Over 22 Different Sections. The Exercises Are Presented In A Simple Language With Explanatory Diagrams And A Brief Recapitulation Of Their Theory And Principle.The Exercises Are Selected By Keeping In Mind The Easy Availability Of Cultures, Culture Media And Equipment. Appendices At The End Of The Manual Provide A Reference To The Source For Obtaining Cultures Of Microbes, Culture Media And Preparation Of Various Stains, Reagents And Media In The Laboratory And Classification Of Procaryotes According To The First And Second Editions Of Bergey Is Manual Of Systematic Bacteriology.This Book Would Be Useful For The Undergraduate And Postgraduate Students, Teachers And Scientists In Diverse Areas Including The Biological Sciences, The Allied Health Services, Environmental Science, Biotechnology, Agriculture, Nutrition, Pharmacy And Various Other Professional Programmes Like Milk Processing Units, Diagnostic (Clinical) Microbiological Laboratories And Mushroom Cultivation At Small Or Large Scales.

Plant Tissue Culture Development and Biotechnology

Author: Robert N. Trigiano
Publisher: CRC Press
ISBN: 1439896143
Format: PDF, ePub
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Under the vast umbrella of Plant Sciences resides a plethora of highly specialized fields. Botanists, agronomists, horticulturists, geneticists, and physiologists each employ a different approach to the study of plants and each for a different end goal. Yet all will find themselves in the laboratory engaging in what can broadly be termed biotechnology. Addressing a wide variety of related topics, Plant Tissue Culture, Development, and Biotechnology gives the practical and technical knowledge needed to train the next generation of plant scientists regardless of their ultimate specialization. With the detailed perspectives and hands-on training signature to the authors’ previous bestselling books, Plant Development and Biotechnology and Plant Tissue Culture Concepts and Laboratory Exercises, this book discusses relevant concepts supported by demonstrative laboratory experiments. It provides critical thinking questions, concept boxes highlighting important ideas, and procedure boxes giving precise instruction for experiments, including step-by-step procedures, such as the proper microscope use with digital photography, along with anticipated results, and a list of materials needed to perform them. Integrating traditional plant sciences with recent advances in plant tissue culture, development, and biotechnology, chapters address germplasm preservation, plant growth regulators, embryo rescue, micropropagation of roses, haploid cultures, and transformation of meristems. Going beyond the scope of a simple laboratory manual, this book also considers special topics such as copyrights, patents, legalities, trade secrets, and the business of biotechnology. Focusing on plant culture development and its applications in biotechnology across a myriad of plant science specialties, this text uses a broad range of species and practical laboratory exercises to make it useful for anyone engaged in the plant sciences.

Plant Tissue Culture Concepts and Laboratory Exercises Second Edition

Author: Robert N. Trigiano
Publisher: Routledge
ISBN: 1351424033
Format: PDF, ePub, Mobi
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Alternating between topic discussions and hands-on laboratory experiments that range from the in vitro flowering of roses to tissue culture of ferns, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, addresses the most current principles and methods in plant tissue culture research. The editors use the expertise of some of the top researchers and educators in plant biotechnology to furnish students, instructors and researchers with a broad consideration of the field. Divided into eight major parts, the text covers everything from the history of plant tissue culture and basic methods to propagation techniques, crop improvement procedures, specialized applications and nutrition of callus cultures. New topic discussions and laboratory exercises in the Second Edition include ""Micropropagation of Dieffenbachia,"" ""Micropropagation and in vitro flowering of rose,"" ""Propagation from nonmeristematic tissue-organogenesis,"" ""Variation in culture"" and ""Tissue culture of ferns.""It is the book's extensive laboratory exercises that provide a hands-on approach in illustrating various topics of discussion, featuring step-by-step procedures, anticipated results, and a list of materials needed. What's more, editors Trigiano and Gray go beyond mere basic principles of plant tissue culture by including chapters on genetic transformation techniques, and photographic methods and statistical analysis of data. In all, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, is a veritable harvest of information for the continued study and research in plant tissue culture science.

Practical manual for Plant Tissue Culture

Author: Hirenkumar Sherathiya
Publisher: GRIN Verlag
ISBN: 3656549664
Format: PDF, Docs
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Document from the year 2012 in the subject Agrarian Studies, , course: Carrier Oriented Program, language: English, abstract: Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. Different techniques in plant tissue culture may offer certain advantages over traditional methods of propagation. This practical manual has been prepared in response to the necessities of the graduate students as an introduction to the in vitro tissue culture techniques and some molecular aspects.