Plant Tissue Culture

Author: Roberta H. Smith
Publisher: Academic Press
ISBN: 0124159206
Format: PDF, Mobi
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Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems Includes detailed drawings that complement both introductions and experiments Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology Fully updated literature

Plant Tissue Culture

Author: Roberta H. Smith
Publisher: Academic Press
ISBN: 0124159850
Format: PDF, ePub
Download Now
Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems Includes detailed drawings that complement both introductions and experiments Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology Fully updated literature

Plant Tissue Culture

Author: Roberta H. Smith
Publisher: Gulf Professional Publishing
ISBN: 9780126503425
Format: PDF
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History of plant cell culture; Media components and preparation; Contamination; Callus induction; Regeneration and morphogenesis; Woody shrubs and trees; Haploid plantas from anther culture; Embryo rescue; Meristem culture for virus-free plants; In vitro propagation for commercial production of ornamentals; Agrobacterium-mediated transformation of plants.

Experiments in Plant Tissue Culture

Author: John H. Dodds
Publisher: International Potato Center
ISBN: 9780521315166
Format: PDF, ePub, Docs
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The second edition of Experiments in Plant Tissue Culture makes available new information that has resulted from recent advances in the applications of plant tissue culture techniques to agriculture and industry. This comprehensive laboratory text takes the reader through a graded series of experimental protocols and also provides an introductory review of each topic. Topics include: a plant tissue culture laboratory, aseptic techniques, nutritional components of media, callus induction, organ formation, xylem cell differentiation, root cultures, cell suspensions, micropropagation, embryogenesis, isolation and fusion of protoplasts, haploid cultures, storage of plant genetic resources, secondary metabolite production, and quantification of procedures. This volume offers all of the basic experimental methods for the major research areas of plant tissue culture, and it will be invaluable to undergraduates and research investigators in the plant sciences.

Plant Tissue Culture An Introductory Text

Author: Sant Saran Bhojwani
Publisher: Springer Science & Business Media
ISBN: 8132210263
Format: PDF, ePub, Mobi
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Plant tissue culture (PTC) is basic to all plant biotechnologies and is an exciting area of basic and applied sciences with considerable scope for further research. PTC is also the best approach to demonstrate the totipotency of plant cells, and to exploit it for numerous practical applications. It offers technologies for crop improvement (Haploid and Triploid production, In Vitro Fertilization, Hybrid Embryo Rescue, Variant Selection), clonal propagation (Micropropagation), virus elimination (Shoot Tip Culture), germplasm conservation, production of industrial phytochemicals, and regeneration of plants from genetically manipulated cells by recombinant DNA technology (Genetic Engineering) or cell fusion (Somatic Hybridization and Cybridization). Considerable work is being done to understand the physiology and genetics of in vitro embryogenesis and organogenesis using model systems, especially Arabidopsis and carrot, which is likely to enhance the efficiency of in vitro regeneration protocols. All these aspects are covered extensively in the present book. Since the first book on Plant Tissue Culture by Prof. P.R. White in 1943, several volumes describing different aspects of PTC have been published. Most of these are compilation of invited articles by different experts or proceedings of conferences. More recently, a number of books describing the Methods and Protocols for one or more techniques of PTC have been published which should serve as useful laboratory manuals. The impetus for writing this book was to make available a complete and up-to-date text covering all basic and applied aspects of PTC for the students and early-career researchers of plant sciences and plant / agricultural biotechnology. The book comprises of nineteen chapters profusely illustrated with self-explanatory illustrations. Most of the chapters include well-tested protocols and relevant media compositions that should be helpful in conducting laboratory experiments. For those interested in further details, Suggested Further Reading is given at the end of each chapter, and a Subject and Plant Index is provided at the end of the book.

Plant Tissue Culture Development and Biotechnology

Author: Robert N. Trigiano
Publisher: CRC Press
ISBN: 1439896143
Format: PDF, Docs
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Under the vast umbrella of Plant Sciences resides a plethora of highly specialized fields. Botanists, agronomists, horticulturists, geneticists, and physiologists each employ a different approach to the study of plants and each for a different end goal. Yet all will find themselves in the laboratory engaging in what can broadly be termed biotechnology. Addressing a wide variety of related topics, Plant Tissue Culture, Development, and Biotechnology gives the practical and technical knowledge needed to train the next generation of plant scientists regardless of their ultimate specialization. With the detailed perspectives and hands-on training signature to the authors’ previous bestselling books, Plant Development and Biotechnology and Plant Tissue Culture Concepts and Laboratory Exercises, this book discusses relevant concepts supported by demonstrative laboratory experiments. It provides critical thinking questions, concept boxes highlighting important ideas, and procedure boxes giving precise instruction for experiments, including step-by-step procedures, such as the proper microscope use with digital photography, along with anticipated results, and a list of materials needed to perform them. Integrating traditional plant sciences with recent advances in plant tissue culture, development, and biotechnology, chapters address germplasm preservation, plant growth regulators, embryo rescue, micropropagation of roses, haploid cultures, and transformation of meristems. Going beyond the scope of a simple laboratory manual, this book also considers special topics such as copyrights, patents, legalities, trade secrets, and the business of biotechnology. Focusing on plant culture development and its applications in biotechnology across a myriad of plant science specialties, this text uses a broad range of species and practical laboratory exercises to make it useful for anyone engaged in the plant sciences.

Plant Tissue Culture

Author: B. N. Sathyanarayana
Publisher: I. K. International Pvt Ltd
ISBN: 8189866117
Format: PDF, Mobi
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Practices and New Experimental Protocols is being brought out to fill the existing gap in the available literature on plant tissue culture, especially focusing on the aspects of practical procedures and protocols of tissue culture. This book contains important experimental techniques and gives guidance on carrying out hands-on experiences. It has been designed in a simple way, giving all the necessary procedures as a general guideline and also necessary tips to maneuver any problem encountered. These tips are based on the first hand experiences of the author while teaching and researching the techniques of plant tissue culture. A unique feature of this book is the inclusion of several techniques describing the actual protocols experimented and developed with different plant species by different scientists. A substantial number of original colored plates including fluorescence photographs standout the book. This pioneering work is valuable for the students who are looking for fresh outlook and search.

Practical Book of Biotechnology Plant Tissue Culture

Author: Madhavi Adhav
Publisher: S. Chand Publishing
ISBN: 8121932009
Format: PDF, Mobi
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The book starts with an introduction to basic knowledge of instruments which deals with principle, working, uses, limitations and precautions of about ten instruments.Basic Knowledge of precaution of;Culture Media for Bacterial Growth, Plant Tissue Culture and Standard Solutions has been given in simple and easy-to-follow language.The biotechnology exercises such as Plasmid and DNA isolation, DNA size determination, Restriction digestion, PCR, Gus gene assay, RFLP, RAPD, Isolation of bacteria by streak and Pour plate method, Growth characteristics of E.Coli by Plating and Turbidimetric method and the plant tissues culture exercises such as Cell suspension culture, Androgenesis, Somatic embryogenesis, Preparation of plantlet to greenhouse field, have been given in a student friendly manner. Matter for Viva-voce has also been included.

Plant Tissue Culture

Author: Margit Laimer
Publisher: Springer Science & Business Media
ISBN: 3709160405
Format: PDF
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In 2002 the 100th anniversary of the publication on "Culturversuche mit isolierten Pflanzenzellen" by Gottlieb Haberlandt was celebrated. Haberlandt ́s vision of the totipotency of plant cells represents the actual beginning of tissue culture. This book pays homage to a great Austrian scientist and the further development of his ideas. The first part of the book contains a facsimile of the original paper which is a true artistic masterpiece and its first translation into English from 1969. The second and third parts describe Haberlandt ́s life and work and early historical aspects of the development of plant tissue culture. The fourth part of the book contains an overview of important topics of plant tissue culture with the most promising areas of application to date and an outlook into the future. Areas range from micropropagation, production of pharmaceutically interesting compounds, plant breeding, genetic engineering of crop plants, including trees, and cryopreservation of valuable germplasm.

Experiments in Microbiology Plant Pathology and Biotechnology

Author: K. R. Aneja
Publisher: New Age International
ISBN: 9788122414943
Format: PDF, Docs
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Microorganisms Are Living Things Like Plants And Animals But Because Of Their Minute Size And Omnipresence, Performing Experiments With Microbes Requires Special Techniques And Equipment Apart From Good Theoretical Knowledge About Them. This Easy To Use Revised And Updated Edition Provides Knowledge About All The Three I.E., Techniques, Equipment And Principles Involved.The Notable Feature Of This Edition Is The Addition Of New Sections On Bacterial Taxonomy That Deals With The Criteria Used In Identification, Phylogeny And Current System Of Classification Of Procaryotes Based On The Second Edition Of Bergey Manual Of Systematic Bacteriology And The Section One On History Of Discovery Of Events That Covers Chronologically Important Events In Microbiology With The Contribution Of Pioneer Microbiologists Who Laid The Foundation Of The Science Of Microbiology. In The Subsequent Twenty-Two Sections, Various Microbiological Techniques Have Been Described Followed By Several Experiments Illustrating The Properties Of Microorganisms And Highlighting Their Involvement In Practically Every Sphere Of Life.Along With The Cultivation/Isolation/Purification Of Microbes, This Edition Also Contains Exercises Concerning Air, Soil, Water, Food, Dairy And AgriculturalMicrobiology, Bacterial Genetics, Plant Pathology, Plant Tissue Culture And Mushroom Production Technology. This Manual Contains 163 Experiments Spread Over 22 Different Sections. The Exercises Are Presented In A Simple Language With Explanatory Diagrams And A Brief Recapitulation Of Their Theory And Principle.The Exercises Are Selected By Keeping In Mind The Easy Availability Of Cultures, Culture Media And Equipment. Appendices At The End Of The Manual Provide A Reference To The Source For Obtaining Cultures Of Microbes, Culture Media And Preparation Of Various Stains, Reagents And Media In The Laboratory And Classification Of Procaryotes According To The First And Second Editions Of Bergey Is Manual Of Systematic Bacteriology.This Book Would Be Useful For The Undergraduate And Postgraduate Students, Teachers And Scientists In Diverse Areas Including The Biological Sciences, The Allied Health Services, Environmental Science, Biotechnology, Agriculture, Nutrition, Pharmacy And Various Other Professional Programmes Like Milk Processing Units, Diagnostic (Clinical) Microbiological Laboratories And Mushroom Cultivation At Small Or Large Scales.