Quantitative Phase Imaging of Cells and Tissues

Author: Gabriel Popescu
Publisher: McGraw Hill Professional
ISBN: 0071663436
Format: PDF, ePub, Docs
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Cutting-edge quantitative phase imaging techniques and their applications Filled with unique, full-color images taken by advanced quantitative phase imaging (QPI), Quantitative Phase Imaging of Cells and Tissues thoroughly explores this innovative technology and its biomedical applications. An introductory background on optical imaging and traditional optical microscopy is included to illustrate concept development. The book explains how various visualization modalities can be obtained by numerical calculations. This authoritative resource reveals how to take full advantage of the unprecedented capabilities of QPI, such as rendering scattering properties of minute subcellular structures and nanoscale fluctuations in live cells. Coverage includes: Groundwork Spatiotemporal field correlations Image characteristics Light microscopy Holography Point scanning QPI methods Principles of full-field QPI Off-axis full-field methods Phase-shifting techniques Common-path methods White light techniques Fourier transform light scattering (FTLS) Current trends in QPI

Introduction to Optical Microscopy

Author: Jerome Mertz
Publisher: Roberts Publishers
ISBN: 9780981519487
Format: PDF, Docs
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Introduction to Optical Microscopy provides a rigorous and comprehensive overview of the fundamentals of optical microscopy. Starting from basic principles in Fourier optics, partial coherence, 3D imaging theory, and the physics of scattering and fluorescence, Introduction to Optical Microscopy explores a broad range of microscopy techniques. These include classical techniques such as phase contrast, confocal microscopy, etc., and progress to more modern techniques such as holographic microscopy, optical coherence tomography, two-photon microscopy, coherent anti-Stokes Raman scattering microscopy, etc.. The final chapters present a survey of new directions, including structured illumination and superresolution. Introduction to Optical Microscopy is designed to provide a solid theoretical foundation for graduate students or researchers who want to enter the field. While valuable as a reference, it can also serve as a textbook, as it includes a corresponding website that provides problem sets and an instructor's solution manual.

Understanding Biophotonics

Author: Kevin Tsia
Publisher: CRC Press
ISBN: 9814411787
Format: PDF, ePub, Docs
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Biophotonics involves understanding how light interacts with biological matter, from molecules and cells, to tissues and even whole organisms. Light can be used to probe biomolecular events, such as gene expression and protein–protein interaction, with impressively high sensitivity and specificity. The spatial and temporal distribution of biochemical constituents can also be visualized with light and, thus, the corresponding physiological dynamics in living cells, tissues, and organisms in real time. Light can also be used to alter the properties and behaviors of biological matter, such as to damage cancerous cells by laser surgery or therapy, and manipulate the neuronal signaling in a brain network. Fueled by the innovations in photonic technologies in the past half century, biophotonics continues to play a ubiquitous role in revolutionizing basic life science studies as well as biomedical diagnostics and therapies. Advancements in biophotonics in the past few decades can be seen not only in biochemistry and cell/molecular biology, but also in numerous preclinical applications. Researchers around the world are searching for ways to bring biophotonic technologies into real clinical practices, particularly cellular and molecular optical imaging. Meanwhile, emerging technologies, such as laser nanosurgery and nanoplasmonics, have created new insights for understanding, monitoring, and even curing diseases on a molecular basis. This book presents the essential basics of optics and biophotonics to newcomers (senior undergraduates or postgraduate researchers) who are interested in this multidisciplinary research field. With stellar contributions from leading experts, the book highlights the major advancements in preclinical diagnostics using optical microscopy and spectroscopy, including multiphoton microscopy, super-resolution microscopy, and endomicroscopy. It also introduces a number of emerging techniques and toolsets for biophotonics applications, such as nanoplasmonics, microresonators for molecular detection, and subcellular optical nanosurgery.

Handbook of Optical Sensing of Glucose in Biological Fluids and Tissues

Author: Valery V. Tuchin
Publisher: CRC Press
ISBN: 9781584889755
Format: PDF, ePub
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Although noninvasive, continuous monitoring of glucose concentration in blood and tissues is one of the most challenging areas in medicine, a wide range of optical techniques has recently been designed to help develop robust noninvasive methods for glucose sensing. For the first time in book form, the Handbook of Optical Sensing of Glucose in Biological Fluids and Tissues analyzes trends in noninvasive optical glucose sensing and discusses its impact on tissue optical properties. This handbook presents methods that improve the accuracy in glucose prediction based on infrared absorption spectroscopy, recent studies on the influence of acute hyperglycemia on cerebral blood flow, and the correlation between diabetes and the thermo-optical response of human skin. It examines skin glucose monitoring by near-infrared spectroscopy (NIR), fluorescence-based glucose biosensors, and a photonic crystal contact lens sensor. The contributors also explore problems of polarimetric glucose sensing in transparent and turbid tissues as well as offer a high-resolution optical technique for noninvasive, continuous, and accurate blood glucose monitoring and glucose diffusion measurement. Written by world-renowned experts in biomedical optics and biophotonics, this book gives a complete, state-of-the-art treatise on the design and applications of noninvasive optical methods and instruments for glucose sensing.

Biomedical Applications of Light Scattering

Author: Adam Wax
Publisher: McGraw Hill Professional
ISBN: 0071598812
Format: PDF, ePub, Mobi
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Clinical applications include: detecting pre-cancerous and cancerous tissue states; characterizing cell and tissue properties for identifying disease; and assessing the presence and concentration of biochemicals for diagnostic purposes Part of the McGraw-Hill Biophotonics Series

Diagnostic Hematology

Author: Norman Beck
Publisher: Springer Science & Business Media
ISBN: 9781848002951
Format: PDF, ePub, Mobi
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The approach described in this book is different from that in most student texts, and has been very successful in practice, starting almost from scratch, but omitting many of the ‘basics’ such as the details of hematopoiesis, laboratory technology, and so on, which are hardly relevant to the practising clinician and student in the wards, and are primarily of interest to the hematologist and sometimes to the clinical specialist. Considerable emphasis is given to the clinical history and examination, and the interpretation of the clinical patterns thus exposed. Hopefully it will overcome many of the traditional problems experienced in practical diagnostic haematology.

Optofluidics Fundamentals Devices and Applications

Author: Yeshaiahu Fainman
Publisher: McGraw Hill Professional
ISBN: 0071601570
Format: PDF, Mobi
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Cutting-Edge Optofluidics Theories, Techniques, and Practices Add novel functionalities to your optical design projects by incorporating state-of-the-art microfluidic technologies and tools. Co-written by industry experts, Optofluidics: Fundamentals, Devices, and Applications covers the latest functional integration of optical devices and microfluidics, as well as automation techniques. This authoritative guide explains how to fabricate optical lab-on-a-chip devices, synthesize photonic crystals, develop solid and liquid core waveguides, use fluidic self-assembly methods, and accomplish direct microfabrication in solutions. The book includes details on developing biological sensors and arrays, handling maskless lithography, designing high-Q cavities, and working with nanoscale plasmonics. Research outcomes from the DARPA-funded Center for Optofluidics Integration are also discussed. Discover how to: Work with optofluidic sources, lenses, filters, switches, and splitters Use dielectric waveguiding devices to input, move, and manipulate fluids Integrate colloidal crystals and fibers with microfluidic systems Develop bio-inspired fluidic lens systems and aspherical lenses Deploy miniaturized dye lasers, microscopes, biosensors, and resonators Analyze microfluidic systems using flow injection and fluorescent spectroscopy Build optofluidic direct fabrication platforms for innovative microstructures Accomplish optofluidic liquid actuation and particle manipulation

Photonica2015

Author: Suzana Petrović
Publisher: Institut za nuklearne nauke VINČA
ISBN: 8673061318
Format: PDF, Mobi
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A Laboratory Manual in Biophotonics

Author: Vadim Backman
Publisher: CRC Press
ISBN: 1498744338
Format: PDF, Docs
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The increasing use of optical techniques for biomedical applications has led to significant interest in biophotonics as a growing field. From its basic concepts to more advanced topics and applications, this book provides a complete guide to biophotonics. The author offers both descriptive and quantitative perspectives as well as a comprehensive overview of major areas of research in the field. The text includes coverage of topics such as light tissue interaction, optical instrumentation, microscopic and macroscopic tissue imaging, tissue spectroscopy, therapeutic applications of optics, and molecular imaging.

Imaging Flow Cytometry

Author: Natasha S. Barteneva
Publisher: Humana Press
ISBN: 9781493933006
Format: PDF, Mobi
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This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.