Quantum Dots for DNA Biosensing

Author: Jun-Jie Zhu
Publisher: Springer Science & Business Media
ISBN: 3642449107
Format: PDF, Docs
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This book provides a broad introduction to all major aspects of quantum dot properties including fluorescence, electrochemical, photochemical and electroluminescence. Such properties have been produced for applications in biosensing, cell tracking, in vivo animal imaging and so on. It focuses on their special applications in DNA biosensing and provides readers with detailed information on the preparation and functionalization of quantum dots and the fabrication of DNA biosensors, using examples to show how these properties can be used in DNA biosensor design and the advantages of quantum dots in DNA biosensing. Further new emerging quantum dots such as metal nanoclusters and graphene dots and their applications in DNA biosensing have also been included.

Cellular and Molecular Toxicology of Nanoparticles

Author: Quaiser Saquib
Publisher: Springer
ISBN: 3319720414
Format: PDF, ePub, Docs
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This edited book is a compilation of findings on the molecular and cellular toxicity of nanoparticles (NPs) in animal cell, human cells, invertebrates. The varied selection of test models will provide better understanding about the horizon of NPs toxicity. Interaction of NPs with cells and its organelles can induce toxicological consequences, including transcriptional and translational alterations, DNA damage, cytotoxicity, oxidative stress, mitochondrial dysfunction and cell death. NPs can get internalized in cells through phagocytosis, macropinocytosis, receptor-mediated endocytosis and passive penetration, which can affect varied cell types. Readers will be benefited with the compilations on basic and molecular facet of NPs toxicity. The chapters will provide a comprehensive information on the state-of-the-art methodologies. The application of toxicogenomic approaches, which is already established in nanotoxicology, has been given special consideration to unravel the toxicodynamics of nanomaterials. Among these approaches, the high-throughput RNA sequencing (RNA-Seq), which is able to build a complete map of transcriptome across different cell types and perturbations upon NPs exposure has been included. The readers are also introduced to the less studied topic on the adsorption of biomolecules (mainly proteins) on the NPs surface, constituting the so-called “biomolecular corona”. The book has been designed for scientists engaged in NPs toxicity research. Nonetheless, it should be of interest to a variety of scientific disciplines including marine biology, environmental pollution, genetics, pharmacology, medicine, drug and food material sciences, consumer products. Also, the compilations will be of interest to the environmental watchdogs, federal regulators, risk assessors and the policy makers.

Biocompatible Graphene for Bioanalytical Applications

Author: Yuwei Hu
Publisher: Springer
ISBN: 3662456958
Format: PDF, Kindle
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This book highlights the latest advances in the use of graphene and bio-compatible-material-decorated graphene to detect various targets (e.g. DNA, RNA, amino acids, peptides, proteins, enzymes, antigens, glucose, DA, AA, UA, ATP, NADH, gas, ions, etc.). It focuses on the specific interaction of these substances with graphene (or modified graphene) and the efficient transduction of the target recognition event into detectable signals via various techniques. Particular emphasis is given to well-designed strategies for constructing graphene-based platforms and target determination. It also covers other bio-analytical applications including cellular imaging, drug delivery and bacteria inhibition, before turning to a discussion of future challenges and prospects of graphene in bio-analytical applications. This book is intended for researchers working in the fields of analytical chemistry, nanomaterials and biomedical engineering. Li Niu is a Professor at the State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences.

MicroRNA Detection and Pathological Functions

Author: Xueji Zhang
Publisher: Springer
ISBN: 3662472937
Format: PDF, Mobi
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This book summarizes microRNA (miRNA) biology in a variety of pathological processes, emphasizing the significant potential applications of miRNA in diagnostics and prognostics, as well as novel drug targets. The conventional techniques used for miRNA detection including standard PCR, Northern blotting, microarray and clone methods are addressed. Recent emerging strategies in miRNA detection and quantification with superior flexibility and adaptability, such as novel molecular biological techniques and locked nucleic acid (LNA) modified probes, as well as nanotechnology-based approaches, are also included. The book also highlights the latest advances in clinical-related miRNA detection methods in living cells, circulating blood and tissue, such as in situ hybridization (ISH) and molecular imaging techniques, which are useful to elucidate the biogenesis and biological function of miRNAs in vivo. Finally, the respective advantages and drawbacks of various detection techniques in this fast-moving field are discussed, along with the challenges and promising new directions. This book offers a valuable resource for analytical chemists, biologists and physicians involved in miRNA research. Dr. Xueji Zhang and Dr. Haifeng Dong are Professors at the School of Chemistry & Biological Engineering, University of Science & Technology Beijing (USTB), China. Dr. Yaping Tian is a Professor at the Department of Clinical Biochemistry, Chinese PLA General Hospital and Military Medical School, China.

Nano Bio Probe Design and Its Application for Biochemical Analysis

Author: Bang-Ce Ye
Publisher: Springer Science & Business Media
ISBN: 3642295436
Format: PDF, Docs
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In this volume, Prof. Ye and his coworkers propose and review the concept of nano-bio probe design for biochemical analysis on the basis of their recent published works. A unique biochemical analysis technology based on fluorescence polarization enhanced by nanoparticles is described here with successful applications in environmental monitoring, rapid and sensitive sensing protease activity and high-throughput screening of inhibitors. Furthermore, they introduce a versatile molecular beacon (MB)-like probe for the multiplex sensing of targets such as sequence-specific DNA, protein, metal ions and small molecule compounds based on the self-assembled biomolecule-graphene conjugates. Besides, some colorimetric and luminescence probes utilizing metal nanoparticles for biochemical applications are also presented, such as chiral enantiomer discrimination and separation, environmental monitoring, clinic diagnosis and etc.

Immunoassays

Author: Arnold Raem
Publisher: Spektrum Akademischer Verlag
ISBN: 9783827416360
Format: PDF, ePub
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Immunoassays, wie ELISA, Western Blots, Immuno-PCR oder Flow-Cytometrie, kommt in der Diagnostik eine große und ständig wachsende Bedeutung zu. Dieses Methodenbuch vermittelt eine gut lesbare, fundierte Einführung in die Grundprinzipien, so dass sich Wissenschaftler der Naturwissenschaften und Medizin wie auch TAs einen ersten, grundlegenden Einblick in die Durchführung der verschiedenen Immunoassays verschaffen können, über deren unterschiedliche Einsatzbereiche, aber auch mit Problemen, Statistik und Validierung konfrontiert werden. Besonders hervorzuheben in diesem Buch sind die akkuraten und funktionierenden Rezepte. Durch ihre tagtägliche Arbeit mit Immunoassays verfügen die Autoren wie auch die Herausgeber über einen reichen Erfahrungsschatz mit diesen Methoden.

Signale Prozesse Systeme

Author: Ulrich Karrenberg
Publisher: Springer-Verlag
ISBN: 3540273476
Format: PDF, Docs
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Buch und CD-ROM bilden ein Lernsystem, welches selbst erforschendes Lernen und die Visualisierung komplexer Vorgänge möglich macht. Das zugrunde liegende didaktische Konzept setzt auf die Visualisierung von Signalen und Prozessen sowie auf die grafische Programmierung signaltechnischer Systeme.

Humangenetik f r Biologen

Author: Werner Buselmaier
Publisher: Springer-Verlag
ISBN: 9783540294795
Format: PDF, ePub, Mobi
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Im Zentrum dieses Lehrbuchs steht die moderne molekularbiologische Entwicklung. Sie besitzt das innovativste Potential innerhalb aller Forschungsgebiete. Neben verschiedenen methodischen Ansätzen präsentieren die Autoren die Ergebnisse des Human Genom Projekts, welches man wohl als das ehrgeizigste Wissenschaftsprojekt der Menschheitsgeschichte bezeichnen kann.

Mathematische Modellierung

Author: Christof Eck
Publisher: Springer-Verlag
ISBN: 3662543354
Format: PDF, ePub, Mobi
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Das Lehrbuch bietet eine lebendige und anschauliche Einführung in die mathematische Modellierung von Phänomenen aus den Natur- und Ingenieurwissenschaften. Leser lernen, mathematische Modelle zu verstehen und selbst herzuleiten und finden eine Fülle von Beispielen, u. a. aus den Bereichen chemische Reaktionskinetik, Populationsdynamik, Strömungsdynamik, Elastizitätstheorie und Kristallwachstum. Die Methoden der Linearen Algebra, der Analysis und der Theorie der gewöhnlichen und partiellen Differentialgleichungen werden sorgfältig eingeführt.