RNA and Protein Synthesis

Author: Kivie Moldave
Publisher: Elsevier
ISBN: 0323142346
Format: PDF, Docs
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RNA and Protein Synthesis is a compendium of articles dealing with the assay, characterization, isolation, or purification of various organelles, enzymes, nucleic acids, translational factors, and other components or reactions involved in protein synthesis. One paper describes the preparatory scale methods for the reversed-phase chromatography systems for transfer ribonucleic acids. Another paper discusses the determination of adenosine- and aminoacyl adenosine-terminated sRNA chains by ion-exclusion chromatography. One paper notes that the problems involved in preparing acetylaminoacyl-tRNA are similar to those found in peptidyl-tRNA synthesis, in particular, to the lability of the ester bond between the amino acid and the tRNA. Another paper explains a new method that will attach fluorescent dyes to cytidine residues in tRNA; it also notes the possible use of N-hydroxysuccinimide esters of dansylglycine and N-methylanthranilic acid in the described method. One paper explains the use of membrane filtration in the determination of apparent association constants for ribosomal protein-RNS complex formation. This collection is valuable to bio-chemists, cellular biologists, micro-biologists, developmental biologists, and investigators working with enzymes.

Contemporary Enzyme Kinetics and Mechanism

Author: Daniel L. Purich
Publisher: Academic Press
ISBN: 1483214222
Format: PDF, Kindle
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Selected Methods in Enzymology: Contemporary Enzyme Kinetics and Mechanism provides an introduction to enzyme kinetics and mechanism at an intermediate level. This book covers a variety of topics, including temperature effects in enzyme kinetics, cryoenzymology, substrate inhibition, enol intermediates enzymology, and heavy-atom isotope effects. Organized into 19 chapters, this book begins with an overview of derivation of rate equations as an integral part of the effective usage of kinetics as a tool. This text then examines the practical aspects of initial rate enzyme assay. Other chapters consider the basic procedures used in making decisions concerning kinetic mechanisms from initial-rate data. This book discusses as well the various aspects of both the theoretical background and the applications. The final chapter deals with the importance of achieving proficiency in formulating quantitative relationships describing enzyme behavior. This book is a valuable resource for students and research workers. Enzymologists and chemists will also find this book useful.

DNA Computing

Author: Japan) International Workshop on Dna-Based Computers 2002 (Sapporo
Publisher: Springer Science & Business Media
ISBN: 3540005315
Format: PDF, Kindle
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This book constitutes the thoroughly refereed post-proceedings of the 8th International Workshop on DNA Based Computers, DNA8, held in Sapporo, Japan, in June 2002. The 30 revised full papers presented were carefully selected during two rounds of reviewing and improvement from an initial total of 68 submissions. The papers are organized in topical sections on self-assembly and autonomous molecular computation, molecular evolution and application to biotechnology, applications to mathematical problems, nucleic acid sequence design, and theory.

Current Catalog

Author: National Library of Medicine (U.S.)
Format: PDF, Docs
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First multi-year cumulation covers six years: 1965-70.