The World of Nano Biomechanics

Author: Atsushi Ikai
Publisher: Elsevier
ISBN: 0444636870
Format: PDF, Mobi
Download Now
The World of Nano-Biomechanics, Second Edition, focuses on the remarkable progress in the application of force spectroscopy to molecular and cellular biology that has occurred since the book's first edition in 2008. The initial excitement of seeing and touching a single molecule of protein/DNA is now culminating in the development of various ways to manipulate molecules and cells almost at our fingertips, enabling live cell operations. Topics include the development of molecular biosensors, mechanical diagnosis, cellular-level wound healing, and a look into the advances that have been made in our understanding of the significance of mechanical rigidity/flexibility of protein/DNA structure for the manifestation of biological activities. The book begins with a summary of the results of basic mechanics to help readers who are unfamiliar with engineering mechanics. Then, representative results obtained on biological macromolecules and structures, such as proteins, DNA, RNA, polysaccharides, lipid membranes, subcellular organelles, and live cells are discussed. New to this second edition are recent developments in three important applications, i.e., advanced AFM-data analysis, high-resolution mechanical biosensing, and the use of cell mechanics for medical diagnosis. Explains the basic physical concepts and mathematics of elementary mechanics needed to understand and perform experimental work on small-scale biological samples Presents recent developments of force-based biosensing Includes novel applications of nano-biomechanics to the medical field

Atomic Force Microscopy in Liquid

Author: Arturo M. Baró
Publisher: John Wiley & Sons
ISBN: 3527327584
Format: PDF, ePub, Docs
Download Now
About 40 % of current atomic force microscopy (AFM) research is performed in liquids, making liquid-based AFM a rapidly growing and important tool for the study of biological materials. This book focuses on the underlying principles and experimental aspects of AFM under liquid, with an easy-to-follow organization intended for new AFM scientists. The book also serves as an up-to-date review of new AFM techniques developed especially for biological samples. Aimed at physicists, materials scientists, biologists, analytical chemists, and medicinal chemists. An ideal reference book for libraries. From the contents: Part I: General Atomic Force Microscopy * AFM: Basic Concepts * Carbon Nanotube Tips in Atomic Force Microscopy with * Applications to Imaging in Liquid * Force Spectroscopy * Atomic Force Microscopy in Liquid * Fundamentals of AFM Cantilever Dynamics in Liquid * Environments * Single-Molecule Force Spectroscopy * High-Speed AFM for Observing Dynamic Processes in Liquid * Integration of AFM with Optical Microscopy Techniques Part II: Biological Applications * DNA and Protein-DNA Complexes * Single-Molecule Force Microscopy of Cellular Sensors * AFM-Based Single-Cell Force Spectroscopy * Nano-Surgical Manipulation of Living Cells with the AFM

Molecular Manipulation with Atomic Force Microscopy

Author: Anne-Sophie Duwez
Publisher: CRC Press
ISBN: 1439809674
Format: PDF, ePub
Download Now
With the invention of scanning probe techniques in the early 1980s, scientists can now play with single atoms, single molecules, and even single bonds. Force, dynamics, and function can now be probed at the single-molecule level. Molecular Manipulation with Atomic Force Microscopy (AFM) presents a series of topics that discuss concepts and methodologies used to manipulate and study single (bio)molecules with AFM. The first part is dedicated to the pulling of single molecules with force spectroscopy to investigate molecular interactions, mechanics, and mechanochemical processes, and the second part to the manipulation, repositioning, and targeted delivery of single molecules on substrates. Single molecule manipulation is an exciting area of research which made important breakthroughs in nanoscience and which could find potential applications in a diverse range of disciplines, including chemistry, biology, physics, material and polymer science, and engineering. New and experienced AFM researchers looking for applications beyond imaging will find a wealth of information in this informative volume.

Neuronal Growth Cones

Author: Phillip R. Gordon-Weeks
Publisher: Cambridge University Press
ISBN: 9780521018548
Format: PDF, ePub
Download Now
'I had the good fortune to behold for the first time that fantastic ending of the growing axon. In my sections of the spinal cord of the three day chick embryo, this ending appeared as a concentration of protoplasm of conical form, endowed with amoeboid movements. It could be compared with a living battering ram, soft and flexible, which advances, pushing aside mechanically the obstacles which it finds in its path, until it reaches the region of its peripheral termination. This curious terminal club, I christened the growth cone.' (Santiago Ramn y Cajal, Recollections of My Life, 1937). In Neuronal Growth Cones, Phillip Gordon-Weeks presents the molecular biology of the behavior of growth cones. The book covers the basic morphology and behavior of growth cones, motility and neurite extension via the growth cone cytoskeleton, pathfinding, intracellular signalling, and synaptogenesis. It is the first detailed, critical analysis of all aspects of growth cone biology.

Drebrin

Author: Tomoaki Shirao
Publisher: Springer
ISBN: 4431565507
Format: PDF, Mobi
Download Now
This book is the first comprehensive review of drebrin, which plays pivotal roles in various cellular events, via forming unique actin cytoskeletons, including synapse formation and in synaptic function. Particularly the loss of drebrin from dendritic spines is used as a marker of dementia in neurological disorders such as Alzheimer’s disease. Since drebrin was first identified by our group in 1985, many studies of drebrin have been done in various fields, including not only molecular biology, biophysics, cell biology, neuroscience, clinical studies, spermatogenesis, immunology, and cancer metastasis, but others as well. The structure of this book facilitates the understanding of the whole picture of studies on drebrin. The volume begins with a general introduction to drebrin, and then the chapters in the second part provide the basic knowledge for further understanding. The third part examines its function in the nervous system, and the fourth part discusses its function in the non-nervous system. This work will appeal to researchers who are interested in cytoskeletal dynamics at membrane-cytoskeletal interface as well as the number of them who use drebrin as a tool, such as a marker of synaptic function or a disease marker. This volume is kept as concise as possible in order to be understood by readers in diverse scientific disciplines.

Scanning Probe Microscopy

Author: Nikodem Tomczak
Publisher: World Scientific
ISBN: 9814324760
Format: PDF, Kindle
Download Now
Scanning Probe Microscopy (SPM) is the enabling tool for nano(bio)technology, which has opened new vistas in many interdisciplinary research areas. Concomitant with the developments in SPM instrumentation and techniques are new and previously unthought-of opportunities in materials nanofabrication and characterisation. In particular, the developments in addressing and manipulating matter at the level of single atoms or molecules, and studies of biological materials (e.g. live cells, or cell membranes) result in new and exciting discoveries. The rising importance of SPM demands a concise treatment in the form of a book which is accessible to interdisciplinary practitioners. This book highlights recent advances in the field of SPM with sufficient depth and breadth to provide an intellectually stimulating overview of the current state of the art. The book is based on a set of carefully selected original works from renowned contributors on topics that range from atom technology, scanning tunneling spectroscopy of self-assembled nanostructures, SPM probe fabrication, scanning force microscopy applications in biology and materials science down to the single molecule level, novel scanning probe techniques, and nanolithography. The variety of topics underlines the strong interdisciplinary character of SPM related research and the combined expertise of the contributors gives us a unique opportunity to discuss possible future trends in SPM related research. This makes the book not merely a collection of already published material but an enlightening insight into cutting edge research and global SPM research trends.

Handbook of Single Molecule Biophysics

Author: Peter Hinterdorfer
Publisher: Springer Science & Business Media
ISBN: 9780387764979
Format: PDF, Kindle
Download Now
This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.

Atomic Force Microscopy in Cell Biology

Author:
Publisher: Academic Press
ISBN: 0080549446
Format: PDF, Mobi
Download Now
This is the first book to cover the history, structure, and application of atomic force microscopy in cell biology. Presented in the clear, well-illustrated style of the Methods in Cell Biology series, it introduces the AFM to its readers and enables them to tap the power and scope of this technology to further their own research. A practical laboratory guide for use of the atomic force and photonic force microscopes, it provides updated technology and methods in force spectroscopy. It is also a comprehensive and easy-to-follow practical laboratory guide for the use of the AFM and PFM in biological research.

Numerical Methods and Advanced Simulation in Biomechanics and Biological Processes

Author: Miguel Cerrolaza
Publisher: Academic Press
ISBN: 0128117192
Format: PDF, ePub, Docs
Download Now
Numerical Methods and Advanced Simulation in Biomechanics and Biological Processes covers new and exciting modeling methods to help bioengineers tackle problems for which the Finite Element Method is not appropriate. The book covers a wide range of important subjects in the field of numerical methods applied to biomechanics, including bone biomechanics, tissue and cell mechanics, 3D printing, computer assisted surgery and fluid dynamics. Modeling strategies, technology and approaches are continuously evolving as the knowledge of biological processes increases. Both theory and applications are covered, making this an ideal book for researchers, students and R&D professionals. Provides non-conventional analysis methods for modeling Covers the Discrete Element Method (DEM), Particle Methods (PM), MessLess and MeshFree Methods (MLMF), Agent-Based Methods (ABM), Lattice-Boltzmann Methods (LBM) and Boundary Integral Methods (BIM) Includes contributions from several world renowned experts in their fields Compares pros and cons of each method to help you decide which method is most applicable to solving specific problems

Aquaporins

Author: Eric Beitz
Publisher: Springer Science & Business Media
ISBN: 3540798854
Format: PDF, Docs
Download Now
The aquaporin field has matured at an exceptionally fast pace and we are at the verge to develop serious strategies to therapeutically modulate aquaporin function directly or via regulatory networks. Key prerequisites are available today: i. a considerable (and growing) number of aquaporin crystal structures for the rational design of inhibitory molecules, ii. elaborate molecular dynamics simulation techniques for theoretical analyses of selectivity mechanisms and docking experiments, iii. comprehensive data on aquaporin immunohistochemistry, iv. aquaporin knockout animals for physiological studies, and v. assay systems for compound library screenings. The structure of this volume on aquaporins follows the points laid out above and thus covers the developments from basic research to potential pharmacological use. Situated between pharmacology textbooks and recent scientific papers this book provides a timely overview for readers from the fundamental as well as the applied disciplines.