Three Dimensional Confocal Microscopy Volume Investigation of Biological Specimens

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Publisher: Academic Press
ISBN: 0323138969
Format: PDF, Mobi
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The integration of confocal microscopy and volume investigation has led to an unprecedented ability to examine spatial relationships between cellular structure and function. The goal of this book is to familiarize the reader with these new technologies and to demonstrate their applicability to a wide range of biological and clinical problems. Volume investigation Three-dimensional reconstruction Fluroescent probe design Biological applications of confocal microscopy, including calcium imaging, receptor movement, and diagnostic pathology Confocal data display and analysis Twenty-eight pages of color

Fluorescence Probes in Oncology

Author: Elli Kohen
Publisher: World Scientific
ISBN: 178326215X
Format: PDF, ePub, Docs
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This unique book provides a comprehensive description of fluorescence probes and the methodology for the study and diagnostics of oncology. The material is drawn directly from the work of pioneer researchers in cell biology and pathology, and offers a perspective of their most crucial investigations and lifetime experiences; it also opens new horizons on future developments in fundamental methods and diagnostics relevant to cellular physiopathology. Researchers in cell pathology have contributed a broad range of spectral and fluorescence images which most appropriately supplement the information derived from Virchow style microscope slides (these still remain valid after more than 150 years, and a considerable body of knowledge and interpretation can be built around them). The text contains about 100 colour pictures, adding great value to the book. Contents:The Nature of LightIntroduction to PhotophysicsBasic Approaches to the Experimental Study of Chemical and Biological LuminescenceMethods and InstrumentationNew Methods. Theoretical Basis and Potential Applications to BiologyFluorescent ProbesApplications of Fluorescence Techniques to Study Biological Processes in Normal and Pathological CellsCell Physiopathology Readership: Students and researchers in oncology, the life sciences, biology and optics. Keywords:Chemiluminescence;Bioluminescence;Green Fluorescent Protein (GFP);Microspectrofluorimetry;Fluorescence Resonance Energy Transfer (FRET);Fluorescence Lifetime Imaging (FLIM);Fluorescence In Situ Hybridization (FISH);Multicolor Spectral Karyotyping (SKY);Fourier Interferometric Spectral Imaging;Fluorescence Imaging;Fluorescence Probe;Oncology;Cell Biology;Pathology;Diagnostics

Handbook of Biological Confocal Microscopy

Author: James Pawley
Publisher: Springer Science & Business Media
ISBN: 1461571332
Format: PDF, Mobi
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In 1987 the Electron Microscopy Society of America (EMSA) going to drive important scientific discoveries across wide areas under the leadership of J. P. Revel (Cal Tech) initiated a major of physiology, cellular biology and neurobiology. They had been program to present a discussion of recent advances in light looking for a forum in which they could advance the state of microscopy as part of the annual meeting. The result was three the art of confocal microscopy, alert manufacturers to the lim special LM sessions at the Milwaukee meeting in August 1988: itations of current instruments, and catalyze progress toward The LM Forum, organized by me, and Symposia on Confocal new directions in confocal instrument development. LM, organized by G. Schatten (Madison), and on Integrated These goals were so close to those of the EMSA project that Acoustic/LM/EM organized by C. Rieder (Albany). In addition, the two groups decided to join forces with EMSA to provide there was an optical micro-analysis session emphasizing Raman the organization and the venue for a Confocal Workshop and techniques, organized by the Microbeam Analysis Society, for NSF to provide the financial support for the speakers expenses a total of 40 invited and 30 contributed papers on optical tech and for the publication of extended abstracts.

Cell Imaging Techniques

Author: Douglas J. Taatjes
Publisher: Springer Science & Business Media
ISBN: 1592599931
Format: PDF, ePub
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Cell imaging methodologies have now become essential research tools for a variety of disciplines that traditionally had not relied on them. In Cell Imaging Techniques: Methods and Protocols, distinguished international researchers describe in detail their state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complementary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Timely and highly practical, Cell Imaging Techniques: Methods and Protocols provides researchers and clinicians with a richly useful guide to selecting and performing the best imaging method from a bewildering variety of microscopy-based techniques.

Digital Image Analysis of Microbes

Author: M. H. F. Wilkinson
Publisher: John Wiley & Sons
ISBN: 9780471974406
Format: PDF, Docs
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Providing specific knowledge in the theory of image analysis, optics, fluorescence, and imaging devices in biomedical laboratories, this timely and indispensable volume focuses on the theory and applications of detection, morphometry, and motility measurement techniques applied to bacteria, fungi, yeasts and protozoa.

Cell Biological Applications of Confocal Microscopy

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Publisher: Academic Press
ISBN: 9780080859361
Format: PDF, Mobi
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Cell Biological Applications of Confocal Microscopy instructs researchers on methods of preparing biological material for confocal microscopy. Individual chapters describe the technical problems of imaging structures in thick specimens and useful techniques such as multi-color dye visualization. The book provides specific examples of applications of confocal microscopy to cell biological problems. This volume is an essential guide for students and scientists incell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities. Describes proper specimen preparation for obtaining high-quality data and images Covers pitfalls in imaging different specimens and in employing different dyes Contains detailed protocols accessible to new users Chapters are self-contained units, but describe strategies that can be combined to develop new protocols Techniques are illustrated with full-color plates

Fluorescence Microscopy

Author: Brian Herman
Publisher: Bios Scientific Pub Limited
ISBN: 9780387915517
Format: PDF, Docs
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Fluoresence Microscopy is the revised, updated and expanded second edition of a best-selling introductory text on the use of fluorescence microscopy in cell biology. The book covers the fundamental principles of fluorescence and their application to fluorescence microscopy. A detailed description of the fluorescence microscope and other required equipment is given, too. Applications to immunofluorescence, in situ hybridization, and photomicrography are presented in full, practical detail. Troubleshooting guidance is provided, along with many practical hints, to guide the user through commonly encountered problems.

Optical methods in drug discovery and development

Author: Mostafa Analoui
Publisher: Society of Photo Optical
ISBN: 9780819460332
Format: PDF, ePub, Mobi
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Proceedings of SPIE present the original research papers presented at SPIE conferences and other high-quality conferences in the broad-ranging fields of optics and photonics. These books provide prompt access to the latest innovations in research and technology in their respective fields. Proceedings of SPIE are among the most cited references in patent literature.