Three Dimensional Confocal Microscopy Volume Investigation of Biological Specimens

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Publisher: Academic Press
ISBN: 0323138969
Format: PDF
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The integration of confocal microscopy and volume investigation has led to an unprecedented ability to examine spatial relationships between cellular structure and function. The goal of this book is to familiarize the reader with these new technologies and to demonstrate their applicability to a wide range of biological and clinical problems. Volume investigation Three-dimensional reconstruction Fluroescent probe design Biological applications of confocal microscopy, including calcium imaging, receptor movement, and diagnostic pathology Confocal data display and analysis Twenty-eight pages of color

Fluorescence Probes in Oncology

Author: Elli Kohen
Publisher: World Scientific
ISBN: 178326215X
Format: PDF
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This unique book provides a comprehensive description of fluorescence probes and the methodology for the study and diagnostics of oncology. The material is drawn directly from the work of pioneer researchers in cell biology and pathology, and offers a perspective of their most crucial investigations and lifetime experiences; it also opens new horizons on future developments in fundamental methods and diagnostics relevant to cellular physiopathology. Researchers in cell pathology have contributed a broad range of spectral and fluorescence images which most appropriately supplement the information derived from Virchow style microscope slides (these still remain valid after more than 150 years, and a considerable body of knowledge and interpretation can be built around them). The text contains about 100 colour pictures, adding great value to the book. Contents:The Nature of LightIntroduction to PhotophysicsBasic Approaches to the Experimental Study of Chemical and Biological LuminescenceMethods and InstrumentationNew Methods. Theoretical Basis and Potential Applications to BiologyFluorescent ProbesApplications of Fluorescence Techniques to Study Biological Processes in Normal and Pathological CellsCell Physiopathology Readership: Students and researchers in oncology, the life sciences, biology and optics. Keywords:Chemiluminescence;Bioluminescence;Green Fluorescent Protein (GFP);Microspectrofluorimetry;Fluorescence Resonance Energy Transfer (FRET);Fluorescence Lifetime Imaging (FLIM);Fluorescence In Situ Hybridization (FISH);Multicolor Spectral Karyotyping (SKY);Fourier Interferometric Spectral Imaging;Fluorescence Imaging;Fluorescence Probe;Oncology;Cell Biology;Pathology;Diagnostics

Handbook of Biological Confocal Microscopy

Author: James Pawley
Publisher: Springer Science & Business Media
ISBN: 1461571332
Format: PDF, Docs
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In 1987 the Electron Microscopy Society of America (EMSA) going to drive important scientific discoveries across wide areas under the leadership of J. P. Revel (Cal Tech) initiated a major of physiology, cellular biology and neurobiology. They had been program to present a discussion of recent advances in light looking for a forum in which they could advance the state of microscopy as part of the annual meeting. The result was three the art of confocal microscopy, alert manufacturers to the lim special LM sessions at the Milwaukee meeting in August 1988: itations of current instruments, and catalyze progress toward The LM Forum, organized by me, and Symposia on Confocal new directions in confocal instrument development. LM, organized by G. Schatten (Madison), and on Integrated These goals were so close to those of the EMSA project that Acoustic/LM/EM organized by C. Rieder (Albany). In addition, the two groups decided to join forces with EMSA to provide there was an optical micro-analysis session emphasizing Raman the organization and the venue for a Confocal Workshop and techniques, organized by the Microbeam Analysis Society, for NSF to provide the financial support for the speakers expenses a total of 40 invited and 30 contributed papers on optical tech and for the publication of extended abstracts.

Cell Imaging Techniques

Author: Douglas J. Taatjes
Publisher: Springer Science & Business Media
ISBN: 1592599931
Format: PDF, Kindle
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Cell imaging methodologies have now become essential research tools for a variety of disciplines that traditionally had not relied on them. In Cell Imaging Techniques: Methods and Protocols, distinguished international researchers describe in detail their state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complementary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Timely and highly practical, Cell Imaging Techniques: Methods and Protocols provides researchers and clinicians with a richly useful guide to selecting and performing the best imaging method from a bewildering variety of microscopy-based techniques.

Digital Image Analysis of Microbes

Author: M. H. F. Wilkinson
Publisher: John Wiley & Sons
ISBN: 9780471974406
Format: PDF, Mobi
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Providing specific knowledge in the theory of image analysis, optics, fluorescence, and imaging devices in biomedical laboratories, this timely and indispensable volume focuses on the theory and applications of detection, morphometry, and motility measurement techniques applied to bacteria, fungi, yeasts and protozoa.

Quantitative Imaging in Cell Biology

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Publisher: Academic Press
ISBN: 0124202012
Format: PDF, Kindle
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This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material

Pathways of a Cell Biologist

Author: Shinya Inoué
Publisher: Springer
ISBN: 9811009473
Format: PDF, Docs
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This book is the autobiography of Shinya Inoué, tracing his life from childhood to the present. Though he has made many contributions to science, perhaps the most remarkable one involves the visualization of dynamics in living cells by means of a polarizing light microscope, an innovation that changed the face of cell biology. Addressing readers curious to know why and how he achieved such success, the story begins with a prologue describing the end of World War II and Inoué’s lifelong collaborator, the great cell biologist Katsuma Dan. Following the prologue, the author’s childhood and teenage experiences during World War II are described, before the focus shifts to his scientific career and personal life. The book not only offers important tips for young researchers, it will also help them develop a passion for science.

Cell Biological Applications of Confocal Microscopy

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Publisher: Elsevier
ISBN: 9780080496580
Format: PDF, ePub, Docs
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This volume of the acclaimed Methods in Cell Biology series provides specific examples of applications of confocal microscopy to cell biological problems. It is an essential guide for students and scientists in cell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities. An integrated and up-to-date coverage on the many various techniques and uses of the confocal microscope (CM). Includes detailed protocols accessible to new users Details how to set up and run a "Confocal Microscope Core Facility" Contains over 170 figures

Cell Biology

Author: Julio E. Celis
Publisher:
ISBN: 9780121647285
Format: PDF
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This Second Edition of the highly praised Cell Biology: A Laboratory Handbook brings together new and revised chapters. Each chapter is concisely written and beautifully illustrated, making the attractive four-volume set a worthwhile addition to any desktop, and the up-to-date instructions for biological techniques make this reference the next best thing to having the expert at your side. Dr. Julio Celis and the Associate Editors have drawn on peer review from the scientific community to include 40 percent new material in this much-needed and updated laboratory manual. In one easy to use reference, current and classic protocols are presented in a clear and reader-friendly format that makes this manual a necessity to undergraduate and graduate students as well as technicians and instructors. Key Features * Contains more than 40% new material * Provides cell biologists and other life scientists with the most up-to-date instructions for basic and advanced cell biological techniques, including those at the interface between cell and molecular biology * Features uniform style and editing and includes contributions from world-renowned authorities in their respective fields * Contains information appropriate for a large, diverse, and constantly growing international audience of cell, developmental, and molecular biologists, plus others who need these methods in their laboratory research * Includes color plates throughout the set for easy reference * Designed as the essential lab guide and research reference for the field

Fluorescence Microscopy

Author: Brian Herman
Publisher: Bios Scientific Pub Limited
ISBN: 9780387915517
Format: PDF, ePub, Docs
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Fluoresence Microscopy is the revised, updated and expanded second edition of a best-selling introductory text on the use of fluorescence microscopy in cell biology. The book covers the fundamental principles of fluorescence and their application to fluorescence microscopy. A detailed description of the fluorescence microscope and other required equipment is given, too. Applications to immunofluorescence, in situ hybridization, and photomicrography are presented in full, practical detail. Troubleshooting guidance is provided, along with many practical hints, to guide the user through commonly encountered problems.